摘要
目的探讨 maspin 和 P53蛋白在胃癌发生、发展中的作用及其与胃癌的相关性,为胃癌的预后判定提供一种新的有效指标。方法采用 Western 印迹、免疫荧光和免疫组织化学(免疫组化)方法,检测32例胃癌患者手术切除的新鲜及石蜡包埋组织中 maspin 蛋白和 P53蛋白的表达。采用免疫组化方法对手术及纤维胃镜切取的病理标本232例进行 maspin 蛋白和 P53蛋白的检测。结果在正常胃黏膜、不典型增生和胃癌组织中,maspin 蛋白在3组间的表达率差异有统计学意义(P<0.05);在不同的组织学类型和不同临床病理分期的表达率差异分别具有统计学意义(P<0.05);在有无淋巴结转移的胃癌组织中的表达率差异有统计学意义(P<0.05);在生存期超过3年和未满3年的患者两组间差异具有统计学意义(P<0.05)。经 Spearman 等级相关检验,P<0.01,maspin 蛋白和 P53蛋白呈负相关。结论 maspin 蛋白和 P53蛋白的表达在胃癌中呈负相关,两者联合检测有助于胃癌预后的判定。
Objective To study the expression of maspin protein and the correlation between maspin and P53 proteins in gastric carcinoma, so as to provide a new valid index for diagnosis of gastric carcinoma. Methods Western blotting, immunofluorescence test, and immunohistochemistry were conducted in 32 fresh specimens of gastric carcinoma tissue and adjacent normal tissues obtained during operation performed from January to December 2005 and 232 specimens of gastric tissues obtained during operation or fibergastroscopy, including 172 specimens of gastric carcinoma, conducted 1998 - 1999. , to determine the protein expression of maspin and P53. The relationship between the protein expression of maspin and that of P53 was analyzed. Results The positive rates of maspin protein in the specimens of fresh gastrie carcinoma tissues by Western blotting, immunofluorescence test and immunohistoehemistry were 56.3%, 50.0%, and 43.8% respectively without significant differences among these 3 groups （ all P 〉 0. 05）. The positive rates of P53 protein in the specimens of the fresh gastric carcinoma tissues by Western blotting, immunofluorescence test and immunohistochemistry were 46. 9%, 40. 6%, and 37. 5% respectively without significant differences among these 3 groups （ all P 〉 0. 05 ）. The analysis of the 232 specimens of gastric tissues obtained during operation or fibergastroscopy showed that the higher the differentiation of the gastric tissue and the lower the stage of carcinoma the higher the positive rate of maspin protein. The positive rates of maspin protein of the normal gastric mucosa, tissue with atypical hyperplasia, and tissue of gastric carcinoma were 58.3%, 52. 8% , and 40.1% respectively with significant differences among these 3 groups （ all P 〈 0. 05 ）. The positive rate of maspin protein of the tissue with lymph node metastasis was 28.8%, significantly lower than that of the tissues without lymph node metastasis （64.8%, P〈0.05）. The positive rate of maspin protein of the patients surviving for more than 3 years was 63.5%, signitleanfly higher than that of the patients surviving for 3 years or less （ 29.6% , P 〈 0.05 ）. Spearman rank correlation test showed a negative correlation between the protein expression of maspin and that of P53 （P 〈 0.01 ）. Conclusion The expression of maspin protein is negatively correlates with that of P53 protein. Detection of both maspin and P53 can serve as a prognostic predictor of clinical outcome in the patients with gastric carcinoma.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第13期909-912,共4页
National Medical Journal of China
基金
黑龙江省青年科学技术专项资金(QC05C38)
黑龙江省科技厅重点课题(ZJY04-0102)
关键词
胃肿瘤
增生
蛋白质P53
印迹法
蛋白质
Stomach neoplasms
Hyperplasia
Protein P53
Blotting ,western
