摘要
目的比较高原世居藏族和移居汉族胎盘线粒体氧化磷酸化功能,探讨世居高原藏族低氧适应的线粒体机制。方法采用 Clark 电极法检测高原世居藏族和移居汉族胎盘线粒体Ⅲ态呼吸(ST3)、Ⅳ态呼吸(ST4)和呼吸控制率(RCR),计算氧化磷酸化效率(OPR);称量世居藏族和移居汉族胎盘重量和新生儿体重;测量胎盘体积及新生儿身长。结果(1)高原世居藏族胎盘重量、体积和新生儿体重、身长均明显高于移居汉族[分别为0.509kg±0.090 kg vs 0.429kg±0.099 kg(t=0.695,P=0.004)、657.66 cm^3±167.10 cm^3 vs 500.46 cm^3±140.69 cm^3(t=1.834,P=0.001)、3.495kg±0.439 kg vs 2.952 kg±0.388 kg(t=3.365,P=0.004)、49.81 cm±2.56 cm vs 48.10 cm±2.30 cm(t=2.025,P=0.000)];(2)高原世居藏族胎盘线粒体 RCR、ST_3、OPR 明显高于移居汉族[4.22±0.97 vs 3.21±0.37(t=3.232,P=0.004)、(67.78±13.57)nmol O·min^(-1)·mg pro^(-1)vs(41.27±10.49)nmol O·min^(-1)·mg pro^(-1)(t=5.257,P=0.000)、(117.93±32.69)nmol O·min^(-1)·mg pro^(-1)vs(68.94±28.88)nmol O·min^(-1)·mg pro^(-1)(t=2.985,P=0.001)],ST_4组间差异无统计学意义[(16.58±3.53)nmol O·min^(-1)·mg pro^(-1)vs(14.79±4.69)nmol O·min^(-1)·mg pro^(-1)(t=1.069,P=0.297)]。结论高原世居藏族胎盘线粒体氧化磷酸化程度显著高于移居汉族,这提示高原世居藏族在高原低氧环境下较移居汉族具有更强的利用氧的能力,可能是其高原低氧适应的重要机制。
Objective To explore the mitochondrial mechanism of adaptation to hypoxia of Tibetan. Methods The body weights and heights of 32 neonates borne by native Tibetan women and of 20 neonates borne by immigrant Han women were measured immediately after birth. The placentae were collected to measure the weight and volume and placental mitochondria were isolated. The mitochondrial respiratory state HI （ST3）, respiratory state IV （ST4）, and respiratory control ratio （RCR） were measured with Clark electrode and the oxidative phosphorylation rate （OPR） was calculated. Results （ 1 ） The mean body weight of the neonates borne by native Tibetan women was 3. 495 ± 0.493 kg, significantly greater than that of the neonates borne by immigrant Han women （2. 952 ± 0. 388 kg, t = 3. 365, P = 0. 000 ）. The mean body height of the neonates borne by native Tibetan women was 49.81 ± 2.56 cm, significantly greater than that of the neonates borne by immigrant Hart women （48.10±2.30 cm, t =2. 025, P =0.000）. The mean weight of the placentae of the native Tibetan women was 0. 509 ± 0. 090 kg, significantly greater than that of the migrant Han women too （0. 429 ± 0. 099 kg, t = 0. 695, P = 0. 004）, and the mean volume of the placentae of the native Tibetan women was 657.66 ± 167. 10 cm^3 , significantly greater than that of the migrant Han women （500.46 ± 140.69 cm^3, t = 1. 834, P = 0.001 ）. （2） The ST3, RCR, and OPR of the placental mitochondria of the native Tibetan women were 4.22 ± 0.97,67.78 ± 13.57 nmol O · min^-1 · mg pro^-1, and 117.93±32.69 nmol O · min^-1 · mg pro^-1, all significantly higher than those of the migrant Han women （3.21 ± 0.37,41.27 ± 10.49 nmol O · min^-1 · mg pro^-1, and 68.94 ± 28.88 nmol O · min^-1 · mg pro^-1 respectively, t = 3. 232,5. 257, and 2. 985, P = 0. 004, 0. 000, and 0. 001 respectively）, while ST4 showed no significant difference between the two groups （ 16.58 ± 3.53 nmol O · min^-1 · mg pro^-1 vs. 14.79±4.69 nmol O · min^-1 · mg pro^-1, t = 1.069, P =0.297）. Conclusion The mitoehondrial oxidative phosphorylation activity is significantly higher in the native Tibetan than in the immigrant Han population, indicating that Tibetans are able to utilize much more oxygen under hypoxic conditions at high altitude. This may be an important mechanism by which Tibetans adapt well to hypoxic environment at high altitude.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第13期894-897,共4页
National Medical Journal of China
基金
国家自然科学基金(30393131
30300123)
关键词
藏族
线粒体
高海拔
氧化磷酸化
胎盘
Zang nationality
Mitochondria
Altitude
Oxidative phosphorylation
Placenta
