摘要
目的研究严重急性呼吸综合征(SARS)冠状病毒(CoV)相关受体血管紧张素转换酶2(ACE2)和 CD209L 在人8种不同器官组织微血管内皮细胞上的表达,分析两种受体表达水平与SARS 器官病变之间的相关性。方法应用反转录聚合酶链式反应(RT-PCR)、Western 印迹、免疫细胞化学3种方法分析比较 SARS-CoV 相关受体,ACE2、CD209L 在人8种不同器官组织微血管内皮细胞上的表达。结果人的各种器官组织的血管内皮细胞均表达 SARS-CoV 相关受体 ACE2与CD209L,其中 ACE2在肺微血管内皮细胞表达最高,在淋巴内皮细胞表达最低,而 CD209L 在淋巴内皮细胞表达相对较高。结论人的微血管内皮细胞是 SARS-CoV 作用的重要靶点,肺微血管内皮细胞和淋巴内皮细胞的损伤可能分别由两种不同的 SARS-CoV 受体介导。
Objective To investigate the expression of the 2 severe acute respiratory syndrome coronavirus （SARS-CoV） receptors, angiotensin-converting enzyme 2 （ACE2） and CD209L in different human organ/tissue derived microvasculax endothelial cells. Methods Endothelial cells from the microvessels in human brain, lung, hepatic sinoside, fat adipose tissue, adrenal gland, esophagus, lymph nodes, and bone were culture. RT-PCR, Western blotting and immunocytochemistry were used to detect the expression of ACE2 and CD209L receptors. Results Both SARS-CoV receptors of ACE2 and CD209L were expressed in the 8 organ/tissue-derived endothelial cells. The expression of ACE2 receptor was the highest in the human lung microvasculax endothelial cells, and lowest in the lymphatic endothelial cells. The expression of CD209L was relatively higher in the human lymphatic endothelial cells. Conclusion The organ derived microvasculax endothelial cells axe the important target of SARS-CoV. The pathological injury of lung and lymph system induced by SARS-CoV may be mediated respectively by different receptors of SARS-CoV.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第12期833-837,共5页
National Medical Journal of China
基金
国家自然科学基金(30340016)
关键词
内皮
血管
肽基二肽酶A
SARS
Endothelium, vascular
Peptidyl-dipeptidase A
SARS
