摘要
用四种不同萃取方法从小蔓长春花中提取活性吲哚生物碱长春胺。比较各方法的回收率及HPPLC分离情况。
Four different extraction methods(traditional soaking, extracting and obtaining the tctal alkaloids from plant sample; soaking the plant sample sequentially with ethanol and phosphoric acid; soaking the plant sample with 95% ethanol; soaking the plant sample with phosphoric acid) for alkaloids in vinca minor were described. Semi-preparative HPLC was used to separate and determine Vincamino in the above mentioned four extracts. Results obtained showed that the direct phosphoric acid extraction method was the best among the four methods. Advantages and disavantages of the four methods were discussed in detail.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1990年第11期1056-1059,共4页
Chinese Journal of Analytical Chemistry
关键词
长春胺
生物碱
萃取
半制备
HPLC
Extraction of aikalcids
Vinca minor
Vincamine
Semi-preparative high preformance liquid chromatography
