摘要
目的探讨颞叶癫癎大鼠脑内 Sema3A 及其受体 Np1的表达变化在癫癎发病中的作用。方法 SD 大鼠制成颞叶癫癎模型,Neo-Timm 染色证实苔藓纤维出芽,免疫组化和原位杂交技术对致癎后不同时间点内嗅皮质和齿状回的 Sema3A mRNA、Np1 mRNA 和蛋白表达进行分析。结果与对照组比较,颞叶癫癎大鼠海马齿状回内分子层存在苔藓纤维出芽(7 d:0.70±0.42,15 d:1.50±0.52,30 d:2.20±0.41,60 d:2.50±0.51,P<0.05);在匹罗卡品致癎后7 d,实验组内嗅皮质区Sema3A mRNA 的表达(0.3006±0.0675)明显低于对照组(0.4562±0.0457,P<0.01);齿状回内 Np1mRNA(0.2337±0.0358)及蛋白(0.2706±0.0389)的表达亦明显低于对照组(P<0.01)。结论内嗅皮质区 Sema3A mRNA、齿状回内 Np1 mRNA 和蛋白的表达下调,可能参与了苔藓纤维的出芽机制。
Objective To explore the changes of Sema3A and it＇ s receptor Npl in temporal lobe epilepsy （TLE） rat brain and the roles in epileptogenesis mechanism. Methods TLE model was established with male healthy SD rats, in which mossy fiber sprouting （MFS） was verified using Neo-Timm staining method. Sema3A mRNA, Npl mRNA and protein was respectively analyzed by immunohistochemistry and in situ hybridization in the entorhinal cortex （EC） or dentate gyrus （DG） at different time after LiCL-PILO induced TLE. Results There were Mossy fiber sprouting （7 d：0. 70 ± 0. 42,15 d：1. 50 ± 0.52,30 d：2. 20 ± 0. 41,60 d ：2.50 ± 0. 51 ） in DG inner molecular layer （IML） of TLE rat compared with those of controls （P 〈 0. 05 ）; At 7 days after lithium-chloride and pilocarpine induced SE, Sema3A mRNA （0. 3006 ± 0.0675 ） in EC significantly decreased （ P 〈 0. 01 ）, Np1 mRNA （0. 2337 ± 0. 0358 ） and protein （0. 2706 ±0. 0389） expressed in DG of hippocampus reduced remarkably compared with those of controls （P 〈 0. 01 ）. Conclusions The down-regulation of Sema3A mRNA in EC, Npl mRNA and protein in DG maybe contribute to the mechanisms of mossy fiber sprouting.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2007年第3期169-172,共4页
Chinese Journal of Neurology
关键词
癫痫
颞叶
嗅通路
齿状回
苔藓纤维
海马
信号素3A
Epilepsy, temporal lobe
Olfactory pathways
Dentate gyrus
Mossy fibers, hippocampal
Semaphorin-3A
