摘要
目的:了解慢性荨麻疹患者血清抗FcεRⅠα自身抗体的阳性率,探讨慢性荨麻疹患者血清中组胺释放活性与抗FcεRⅠα自身抗体的关系。方法:以FcεRIα融合蛋白(rsFcεRⅠα)为抗原,用ELISA和免疫印迹法检测慢性荨麻疹患者血清中抗FcεRIα自身抗体,并通过抗FcεRⅠα自身抗体对组胺释放活性的影响证实其功能。结果:62例慢性荨麻疹患者中检出抗FcεRⅠα自身抗体阳性者22例(35.5%),其血清引起的组胺释放率平均为26.26%±16.08%;将其血清与rsFcεRⅠα作用后组胺释放率下降为9.14%±5.79%,组胺释放活性明显受到抑制。结论:部分慢性荨麻疹患者血清中的组胺释放活性因子与其血清中的抗FcεRIα自身抗体有关,抗FcεRⅠα自身抗体可能直接活化肥大细胞,释放组胺等血管活性介质,引起荨麻疹的发生。
Objective： To determine the positivity rate of anti - FcεR Ⅰα auto - antibodies in chronic urticaria and to explore the relationship between the histamine - releasing activity and anti - FcεR Ⅰα auto - antibodies. Methods： Sera from 62 patients with chronic urticaria were tested for the presence of anti - FcεR Ⅰα auto - antibodies against rsFcεR Ⅰα antigen using ELISA and Western blotting methods. The function of the auto - antibodies was demonstrated by the influence of FeaR I a auto- antibodies on histamine release. Results： Anti- FcεR Ⅰα auto- antibodies were found in 35.5 % （22/62） of patients with chronic urticaria. The histamine release from mast cells was decreased to 9. 14% ± 5.79% when the sera from these positive patients were incubated with rsFcεR Ⅰα. Conclusion ： In some patients of urticaria, histamine - releasing activity factor, which is related to the circulating functional FcεR Ⅰα antibodies, is responsible for the oceurrence of urticaria by direct activation of master cell and release of blood vessel activating mediators such as histamine.
出处
《中国麻风皮肤病杂志》
2007年第4期280-282,共3页
China Journal of Leprosy and Skin Diseases
