摘要
目的研究口腔疣状癌与口腔鳞癌组织基因的差异表达,探讨口腔疣状癌(oralverrucous carcinoma,OVC)与口腔鳞癌(oral squamous cell carcinoma,OSCC)的基因学基础。方法应用 cDNA 芯片技术对4例 OVC 和4例 OSCC 组织 mRNA 检测,通过芯片杂交、生物信息学处理,找出两者间差异表达基因。结果 BioStarH-40芯片发现差异表达基因593条,差异表达基因占15.2%,其中表达增强283条(显著增强59条),表达降低310条(显著降低98条)。结论 OVC 与 OSCC 基因表达比较,差异有统计学意义,这些差异可能在各自不同的生物学行为中起重要作用。
Objective To determine the difference in the gene expression between human oral verrucous carcinoma（OVC） and oral squamous cell carcinoma（OSCC）. Methods cDNA chip was used to detect the mRNA of cancer tissue from 40VC and 40SCC. After profile bloted and handled by bioinformation, the gene expression of these two kinds of lesions was examined. Results Using the BioStarH-40 profile, 593 different expression of genes was found. The rate of different genes was 15. 2%, of which the exprssion of 283 genes increased （59 genes significantly increased） and 310 genes decreased （ 98 genes significandy decreased ） in OVC tissue than that in OSCC. Conclusions The gene expression of OVC and OSCC was different, which many contribute to the different biological behavior of these two kinds of lesions.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2007年第4期229-230,共2页
Chinese Journal of Stomatology
基金
湖南省自然科学基金(03JJY3045)
关键词
癌
疣状
癌
鳞状细胞
基因表达
Carcinoma,verrucous
Carcinoma, squamous cell
Gene expression
