摘要
利用计算机分子模拟系统模建了CNTFRα的三维结构,并对CNTF与CNTFRα结合的关键部位进行分析,同时综合了同源比较的结果及CNTF分子自身的特点,设计了2个CNTF的突变体A和B.表达纯化后得到目的蛋白,TF-1细胞增殖实验的结果表明,2个突变体蛋白的比活性均达到106U/mg的水平,且A的活性大于B的活性.上述结果初步表明设计的合理性,为其进一步的开发和应用奠定了基础.
In order to present clues to find safer and more efficient ciliary neurotrophic factor（CNTF）, computer molecular modeling system was used to gain the three-dimension structure of CNTFRα.Then through analysing the CNTF/CNTFRα crucial sites to its bioactivity combined with the homologous sequences comparison and CNTF molecular characters, two CNTF mutants were designed. The mutants＇ bioactivity were tested by the experiment of TF-1 proliferation. The two mutants＇ bioactivity reached 10^6 U/mg level after expression and purification and the bioactivity of A is higher than that of B. The results proved primarily that the design was reasonable and provided premise and basement for CNTF＇s development and application.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2007年第4期408-411,共4页
Progress In Biochemistry and Biophysics
基金
国家高技术研究发展计划资助项目(2003AA2Z3480)~~
