摘要
将获得的拟南芥黑芥子酶结合蛋白PBP1基因表达载体pTYB2-PBP1导入大肠杆菌ER2566细胞,经IPTG诱导,SDS-PAGE电泳显示,PBP1-CBD融合蛋白获得了较好的表达。进一步对PBP1蛋白进行分离纯化,SDS-PAGE电泳结果显示分离纯化效果较好,所获得的纯化的PBP1蛋白可进一步用于研究PBP1与MBP6的酵母双杂交技术。
In order to obtain purified PBP1 protein to study the reciprocity between PBP1 protein and MBP6, the expressive vector pTYB2-PBP1 of the PBP1 gene of myrosase-binding protein from Arabidopsis was transformed into E. coli ER2566. The results by SDS-PAGE showed that the fusion protein PBP1-CBD was expressed well by IPTG induction. The expressive product was separated and purified and the purified protein can be used to study the Yeast Two-Hybrid System technology between PBP1 and MBP6.
出处
《河南农业科学》
CSCD
北大核心
2007年第4期39-41,共3页
Journal of Henan Agricultural Sciences
