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人釉原蛋白编码区基因原核表达载体的构建 被引量：2

Construction of the prokaryotic expression vector for human amelogenin gene
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摘要 目的:构建人釉原蛋白(amelogenin,AMG)成熟肽编码区基因的原核表达载体。方法:运用TA克隆技术,将AMG基因片断插入质粒pMD 18-T,通过BamHⅠ和XhoⅠ双酶切后,T4 DNA连接酶连接目的基因AMG与原核表达质粒pGEX-4T-1,最终获得重组原核表达载体pGEX-4T-1/AMG。结果:对重组质粒pMD 18-T/AMG和pGEX-4T-1/AMG进行酶切和测序鉴定,酶切电泳图和测序结果与预期结果一致。结论:成功构建了人釉原蛋白(AMG)成熟肽编码区基因的原核表达载体。 PURPOSE： To construct the prokaryotic expression vector for the geue encoding human amelogenin mature poptide, METHODS： The DNA sequence encoding human amelogenin was inserted into the plasmid pMD 18-T by TA clone technique, pMD 18-T/AMG was digested by BamH Ⅰ and Xho Ⅰ . Target DNA was cloned into Prekaryotic expression plasmid pGEX-4T-1 by T4 DNA ligase RESULTS： Double endonuclease digestion electrophoresis and DNA sequence analysis was conducted to identify the recombined vector pMD 18-T/AMG and pGEX-4T-1/AMG. The results were consistent with the anticipation. CONCLUSION： The prekaryotic expression vector for the gene encoding human amelogenin mature peptide is constructed successfully. Supported by Guangdong Provincial Major Program for Famous Doctors（Grant No.2004-29）, Medical Scientific Research Foundation of Guangdong Province （Grant No.A2006113）and Excellent Youth Foundation of Henan Province（Grant No.512001000）.
作者 张雪洋 赵华 胡飞 赵红宇 章锦才
机构地区 首都医科大学口腔医学院 广东省口腔医院
出处 《上海口腔医学》 CAS CSCD 2007年第1期81-84,共4页 Shanghai Journal of Stomatology
基金 广东省名医工程研究项目(2004-29) 广东省医学科研基金(A2006113) 河南省杰出青年科学基金(512001000)~~
关键词 人釉原蛋白 原核 载体 Human Amelogenin C, ene Vector
分类号 R78 [医药卫生—口腔医学]
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参考文献8

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  • 2Popowics T,Foster BL,Swanson EC,et al.Defining the roots of cementum formation[J].Cells Tissues Organs,2005,181:248 -257.
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二级参考文献24

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共引文献9

同被引文献19

  • 1Fukae M, Kanazashi M, Nagano T, et al. Porcine sheath proteins show periodontal ligament regeneration activity. Eur J Oral Sci, 2006, 114( Suppl 1) : 212-218.
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  • 4Boyan BD, Weesner TC, Lohmann CH, et al. Porcine fetal enamel matrix derivative enhances bone formation induced by demineralized freeze dried bone allograft in vivo. J Periodontol, 2000, 71(8) : 1278-1286.
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引证文献2

二级引证文献1

上海口腔医学
2007年 第1期

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