摘要
以柴胡皂苷a为指标,采用高效液相色谱法测定洗脱液中柴胡皂苷a的含量,考察D-101大孔吸附树脂对柴胡总皂苷的吸附性能和洗脱条件,以柴胡提取液15 mL(0.2 g药材/mL)上柱,70%的乙醇洗脱,柴胡总皂苷洗脱率为71.83%.且除杂能力强.
The absorptive characterstics and elutive parameters of D-101 in the process was studied by using HPLC to monitor saikkosaponins a. The concentrate of crude flavonoids in Bupleurum Chinese DC. 15 mL （0. 2 g medicine/mL） was pillared, then the destilled water, 30%, 50%, 70%, 80%, 90% concentration of ethanol solution were used for the 70% ethanol to total saponins in Bupleurum Chinese DC. elution rate was 71.83%.
出处
《分析测试技术与仪器》
CAS
2007年第1期22-24,共3页
Analysis and Testing Technology and Instruments
关键词
高效液相色谱
大孔树脂
柴胡总皂苷
富集纯化
HPLC
D-101 macroporas resin
total saponinsin from Bupleurum Chinese
enrichment and purification
