摘要
目的探讨趋化因子受体CCR7在胃癌组织中的表达及其临床意义。方法采用RT-PCR及West-ern blot法检测32例胃癌及其癌旁对照组织中CCR7的表达。结果CCR7 mRNA在胃癌组织中的表达水平为癌旁对照组的1.66倍(P<0.01),且其表达水平与肿瘤TNM分期(P<0.05)、浸润深度(P<0.05)及淋巴结转移有关(P<0.05);Western blot结果显示CCR7蛋白在胃癌组织中的表达水平为癌旁对照组的2.3倍(P<0.01),且高表达于肿瘤分期较晚(P<0.05)、分化较差(P<0.05)、浸润较深(P<0.05)、有淋巴结转移(P<0.05)和远处转移(P<0.05)的组织。结论趋化因子受体CCR7可能与胃癌的发生、发展、侵袭、转移有关。
Objective To examine the expression of the chemokine receptor CCR7 in gastric carcinoma and study its clinical significance. Methods The expressions of chemokine receptor CCR7 mRNA and CCR7 protein in 32 specimens of gastric carcinoma and the adjacent normal tissue were examined by reverse transcription - polymerase chain reaction （ RT - PCR） and Western blot. Results The average mRNA expression levels of CCR7 in gastric carcinoma tissue was 1.66 times that of the adjacent normal tissue （P 〈 0.01 ）. The expression of CCR7 mRNA was found to be relevant to TNM stage, depth of invasion and lymph node metastasis （ P 〈 0.05 ）. The protein expression level of CCR7 in gastric carcinoma tissue was 2.3 times that of the adjacent normal tissue （ P 〈 0.01 ）. The protein expression level of CCR7 in stage Ⅲ - Ⅳ was proportionally higher than in stage Ⅰ - Ⅱ （ P 〈 0.05 ）. The protein expression level was significantly higher in more invasive tumors （P 〈 0.05）, and in the cancers with lymph node metastasis and distant metastasis （ P 〈 0.05）. Conclusion The expression of chemokine receptor CCR7 in gastric carcinoma is related to the development and degree of malignancy of the cancer.
出处
《徐州医学院学报》
CAS
2007年第4期236-239,共4页
Acta Academiae Medicinae Xuzhou
关键词
趋化因子受体
CCR7
胃肿瘤
侵袭
转移
chemokine receptor
CCR7
stomach neoplasms
invasion
metastasis
