摘要
目的探讨表皮生长因子受体（EGFR）反义重组腺病毒AdE5联合放射线对乳腺癌细胞移植瘤的作用。方法人乳腺癌细胞MDA-MB-231接种于裸鼠右侧背部皮下形成肿瘤后，以EG- FR反义重组腺病毒AdE5联合放射线处理，观察其对肿瘤体积、瘤内Ki-67表达、微血管生成（MVD值）的影响。结果AdE5瘤内注射可抑制肿瘤生长，AdE5联合照射组肿瘤相对生长率明显低于其他对照组（P＜0．01）。免疫组化分析发现，AdE5联合放射线后肿瘤内Ki-67的表达及MVD也受到显著抑制（P＜0．01）。结论在此乳腺癌模型上，EGFR反义重组腺病毒AdE5可有效地抑制乳腺癌细胞的在体肿瘤生长，并可以提高乳腺癌细胞移植瘤的放射敏感性。
Objective To investigate the antitumor effects of recombinant epidermal growth factor receptor（EGFR） antisense adenovirus（AdE5） plus radiation on breast cancer cells in the mice. Methods Human breast cancer MDA-MB-231 cells were injected subcutaneously into the right flank of nude mice. Intra-tumoral injections of AdE5 alone or combined with ionizing radiation were given to observe the effects on tumor growth, intra-tumoral Ki-67 expression and microvessel density（MVD）. Results AdE5 inhibited tumor growth, and the tumor relative growth index decreased more severely when it was combined with ionizing radiation, as compared with other control groups （ P 〈 0. 01 ）. Immunohistochemical study showed that intra-tumoral Ki-67 expression and MVD were significantly inhibited by AdE5 plus ionizing radiation, as compared with other control groups （ P 〈 0.01 ）. Conclusion Recombinant EGFR antisense adenovirus is effective for inhibition of breast cancer cells growth in vivo, and for increase of the anti-tumor effect of ionizing radiation, as shown in this breast cancer model.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2007年第2期136-139,共4页
Chinese Journal of Radiation Oncology
基金
国家自然科学基金资助（30171066）
关键词
表皮生长因子受体
反义
腺病毒
乳腺癌
放射敏感性
Epidermal growth factor receptor
Antisense
Adenovirus
Breast cancer
Radiosensitivity
