摘要
利用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）方法对６５例分泌性中耳炎患者的中耳积液进行肠道病毒检测，１５例肠道病毒为阳性，阳性率为２３．１％（１５／６５）。发病２周内肠道病毒阳性率为３８．２％（１３／３４）；发病一个月以上肠道病毒阳性率为６．４％（２／３１），二者差异显著（Ｐ＜０．０１）。结果提示ＲＴ－ＰＣＲ方法是一种敏感性高、特异性强的病毒检测方法，适合于中耳积液中肠道病毒的检测，肠道病毒在分泌性中耳炎发生过程中，特别是发生的早期阶段可能发挥重要作用。
Abstract Sixty-five specimens of middle ear effusion in patients with secretory otitis media were studied to detect enterovirus with RT- PCR methd. Enterovirus- positive rates was 23.1%(15/65)in all specimens,38.2%(13/34)in specimens with otological symptoms less than 2weeks.5%(2/31)in specimens with otological symptoms more than l month,respec-tively The difference between the latter two rates was statistically significant(P<0. 01)The results show that RT-PCR methed is high-sensitive,high-specific methOd for en-teroviral detection in middle ear effusion,and enterovirus may play an important role in the pathogenesis of secretory otitis media,especially in the early stage;
出处
《耳鼻咽喉（头颈外科）》
1996年第2期77-79,共3页
Chinese Arch Otolaryngology-Head Neck Surg
