摘要
目的:探讨三七复合有效成分在子宫内膜细胞炎症过程中对NF-κBp65表达和活性变化及其TNF-α、IL-1β含量变化的影响。方法:应用WB,ICC,EMSA方法分析子宫内膜细胞LPS炎症模型组、三七复合有效成分组、宫血宁制剂组及正常对照组NF-κBp65的表达情况以及其活性的动态变化;应用ELISA法测定TNF-α、IL-1β的含量。结果:子宫内膜细胞炎症过程中细胞核内出现NF-κBp65的高水平表达(P<0.01),TNF-α、IL-1β的含量随炎症发展而逐渐上升(P<0.01),三七复合有效成分能有效降低炎症因子TNF-α、IL-1β的含量,降低NF-κBp65的转录及蛋白表达量,抑制NF-κBp65的核因子DNA结合活性。结论:三七复合有效成分能促使炎症细胞转归,有效保护LPS对培养细胞的损伤,在长效持续性保护细胞形态完整性方面优于宫血宁制剂。
Objective: To investigate the change of nuclear factor κBp65 and TNF-α, IL-1β expression in the inflammatory model of human endometrial cell affected by the compound components from panax notoginseng. Methods: EMSA, ICC and WB were used to detect the activity of NFκBp65 , the ELISA were used to detect the concentration of TNF-α and IL-1β. Results: Nuclear translocation of NF-κBp65 appeared in the progression of infection model of human endometrial cell, the activity of NF-κBp65 were increased obviously in nuclear, and the concentration of TNF-α, IL-1β also increased. The compound components from panax notoginseng could inhibit the expression of TNF-α, IL-1β and NF-κBp65 , the transcription of NF-κBp65, and the DNA binding activity of NF-κBp65 . Conclusion: The compound components from panax notoginseng can reduce the intensity of inflammation and especially have predominance on protection of integrality of endometrial cell contrasted with gongxuening capsule.
出处
《中国中医药科技》
CAS
2007年第2期113-115,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金资助项目No.30271633
湖南省社会发展重大项目No.OISSY1002-4
