摘要
目的:探讨载脂蛋白(APO)A5-1131T>C基因多态性与冠心病的关系。方法:采用聚合酶链反应—限制性片段长度多态性结合聚丙烯酰胺凝胶电泳技术,对186例冠心病患者(冠心病组)及268名健康对照者(对照组)的APOA5-1131T>C基因多态性进行检测,同时采用酶法测定研究对象的血脂水平。结果:冠心病组与对照组的APOA5-1131T>C基因型分布有显著性差异,冠心病组的c等位基因频率明显高于对照组(0．422比0．321,P<0．05)。不同APOA5-1131T>C基因型的血清TG水平有明显差异,TC型和CC型的TG水平高于TT型(P均<0．01),但TC型与CC型之间无显著性差异(P>0．05);在冠心病组患者中,C等位基因携带者(TC+CC型)的TG水平也明显高于非C携带者(TT型1．73±0．95 mmol／L比1．46±0．86 mmol／L,P<0．05);而其他血脂指标各基因型之间无统计学差异(P>0．05)。单因素logistic回归分析显示APOA5 TC+CC基因型是冠心病的危险因素[风险比值(OR)=1．520,95％可信区间(95％CI)1．044—2．215,P=0．029],但多因素logistic回归分析校正体重指数、高血压、糖尿病和高密度脂蛋白胆固醇等影响因素之后,APOA5 TC+CC基因型不是冠心病的独立危险因素(OR=1．331, 95％CI 0．634—2．794,P=0．449)。结论:冠心病患者的APOA5-1131C等位基因频率显著高于健康人群,APOA5-1131T>C基因变异与血清TG水平增高密切相关,APOA5-1131T>C基因变异可能与冠心病危险性增加有关,但作为冠心病的独立危险因素尚需更大样本人群研究证实。
Objective：To investigate the correlation between apolipoprotein （APO） A5-1131T 〉 C polymorphism and coronary heart disease （CHD）.
Methods：Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and polyacrylamide gel eletrophoresis （PAGE） were used to analyze genotypic polymorphism in 186 patients with CHD and 268 healthy controls. The levels of serum lipid profiles were also measured by enzymatic methods.
Results：The APOA5-1131T 〉 C genotype distribution in CHD group and the control group showed a significant difference. The frequency of the -1131C allele in patients with CHD was significantly higher than that of the controls （0. 422 vs. 0. 321 ,P〈0. 05）. The serum TG levels among three genotype groups showed a significant difference. The TG levels in type TC（ 1.50±0. 78 mmol/L） and type CC（ 1.73±1.22 mmol/L） were signifieantly higher than in type TT（ 1.29 ±0. 74 mmol/L）（ all P 〈0. 05 ）, but there was no significant difference between type TC and type CC（P 〉0. 05）. Compared with nonearriers （type TC ＋ CC）, C carriers （type Tr） also had a higher TG levels in CHD group （ 1.46 ± 0. 86 mmol/L vs 1.73± 0. 95 mmol/L, P 〈 0. 05 ）. The levels of TC, HDL-C and LDL-C showed no statistical differences between various genotypes （ P 〉 0. 05 ）. Unadjusted logistic regression analysis indicated that TC ＋ CC genotype of A5-1131T 〉 C had an increased CHD risk （ OR = 1. 520,95% CI 1. 044-2. 215 ,P =0. 029）. But the analysis did not show that TC ＋ CC genotype was independently associated with an increased risk of CHD （ OR = 1. 331,95% CI 0. 634-2. 794,P =0. 449） after adjusted for BMI, presence of hypertension or diabetes and HDL-C levels.
Conclusion ： The frequency of APOA5-1131C allele in patients with CHD was significantly higher than that of the healthy individuals. The APOA5-1131T 〉 C variant was significantly associated with increased serum TG levels. The APOA5-1131T 〉 C variant may be associated with an increased risk of CHD. However, as an independent risk factor for CHD needs further study.
出处
《中国循环杂志》
CSCD
北大核心
2007年第1期4-8,共5页
Chinese Circulation Journal
关键词
冠状动脉疾病
多态性
限制性片段长度
载脂蛋白类
甘油三酯
Coronary heart disease
Polymorphism, restriction fragment length
Apolipoprotein
Triglyceride
