摘要
目的:研究环氧合酶(COX)-2抑制剂美洛昔康对肝癌细胞HepG2的生长抑制作用及其作用机制。方法:MTT法观察美洛昔康在不同浓度和作用时间下对细胞增殖的抑制作用;免疫细胞化学染色检测细胞增殖核抗原(PCNA)的表达;DNA原位末端标记(TUNEL)法和流式细胞术(FCM)检测细胞凋亡。结果:美洛昔康可抑制人肝癌细胞HepG2增殖;TUNEL及FCM检测结果均显示美洛昔康可诱导HepG2细胞凋亡。结论:美洛昔康可通过抑制细胞增殖和诱导凋亡2种途径抑制人肝癌细胞HepG2的生长。
OBJECTIVE： To study the inhibitory effects of a COX- 2 inhibitor meloxicam on the growth of human liver cancer cell line HepG2 and potential mechanism. METHODS： The effect of meloxicam on the growth of HepG2 cells was evaluated by MTT assay. The expression of proliferating cell nuclear antigen （PCNA） was assessed by immunocytochemistry method. Apotosis index （AI） was counted by terminal deoxynucleotidly transferase - mediated UTP nick end - labelling （TUNEL） . The apoptosis rate （AR） was quantified by flow cytometry （FCM） . RESULTS： （1） Meloxicam inhibited the proliferation of HepG2 cells. （2）The results of TUNEL and FCM confirmed that meloxicam could induce the apoptosis of HepG2 cells. CONCLUSIONS： Meloxicam suppresses the growth of HepG2 cells by inhibiting proliferation and inducing apoptosis.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第10期744-746,共3页
China Pharmacy
