摘要
采用双侧颈总动脉和椎动脉夹闭加全身降压建立的脑缺血模型研究钙通道拮抗剂尼莫地平对犬完全性脑缺血后脑血流量（CBF）、脑匀浆三磷酸腺苷（ATP）和神经机能恢复的影响。全麻药为硫喷妥钠20mg／kg和琥珀酰胆碱0．1g静注，气管插管机械控制呼吸。脑缺血15分钟后（恢复灌流后），缺血对照组（B组和C组，犬各6只）动物生理盐水静脉滴入，尼莫地平治疗组以尼莫地平20μg／kg，半量立即静注，另半量30分钟内静脉滴入（D组和E组，犬各6只）。其中C组和E组动物颅骨开窗备取脑皮层组织。复灌后120分钟治疗组动物的CBF和脑ATP比对照组动物显著增高，神经缺陷积分和存活数比对照组略好，但差异无显著性。结果表明，尼莫地平有助于犬脑缺血15分钟后CBF的改善和脑ATP的恢复，但尚不足以明显改善神经机能和提高存活率。
This study tested the hypothesis that nimodipine will improve post-ischemic neurologic outcome when administered after a period of complete cerebral ischemia. Anesthesia for the preischemic surgical preparation was induced and maintained with sodium pentothal 20mg/kg intravenoues injection. Succinylcholine 100 mg infusion was given to facilitate tracheal intubation. Complete cerebral ischemia was produced by ligation of bilateral common carotid and vertebral arteries under controlled hypotention. Fifteen minutes of complete cerebral ischemia was foL lowed by an infusion of nimodipine 20μg/kg intravenous injection over 30 minutes in treated animals group D n=6), and group E n= 6 and normal saline injection in control animals group B , n= 6 and group C n = 6). The dorsal skull of animals in group C and E was opened for brain tissue obtaining. By 120 minutes after reperfusion, cerebral blood flow (CBF) and brain adenosine triphosphate (ATP) level of treated animals were significantly higher than that of control animals. After 24 hours two of six treated animals survived but all of control animals died. The difference between the two groups of animals in survivors and neurologic deficit scores (NDS) was not significant. We conclude that nimodipine had a beneficial effect on the recovery of CBF and cerebral ATP level, but that dose of nimodipine could not markedly improve animals neurologic function.
出处
《急诊医学》
CSCD
1996年第3期135-138,共4页
关键词
脑缺血
脑血流量
神经缺陷积分
尼莫地平
Complete cerebral ischemia
Calcium entry blocker
Cerebral blood flow
Neurologic deficit score
