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热休克蛋白47在糖尿病大鼠皮肤创口愈合过程中的表达 被引量：1

Expression of 47 kDa Heat Shock Protein during Wounds Healing in Diabetic Rat Skin
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摘要 目的:使用糖尿病大鼠模型探讨热休克蛋白47(47kDa heat shock protein,HSP47)在糖尿病大鼠皮肤创口愈合过程中的表达水平。方法:在距正常大鼠和糖尿病大鼠头部1cm的背部做一直径1cm的圆形全层皮肤创口,糖尿病大鼠分为不注射组、注射空质粒组和注射重组质粒组,在创口愈合的第3、5、7天分别取对照组和实验组大鼠的创周皮肤,应用免疫组织化学和Western免疫印记法(Western blotting)检测HSP47在各组大鼠皮肤创口愈合过程中的表达水平。结果:正常大鼠皮肤创周组织中HSP47的表达,在愈合过程中呈明显增高的趋势。同正常大鼠相比,糖尿病大鼠皮肤创口愈合缓慢,皮肤创口组织中HSP47的表达明显减少。注射重组质粒pTracer-CMV-HSP47的糖尿病大鼠皮肤创周组织中HSP47的表达增强。结论:HSP47在糖尿病大鼠皮肤创口组织中的低表达与糖尿病大鼠皮肤创口愈合缓慢密切相关。通过体外构建HSP47的重组质粒再注入糖尿病大鼠皮肤创周组织中的方法,可以增强HSP47在体内的表达。 Objective： To investigate the expression of 47kDa heat shock protein（HSP47） during wounds healing in diabetic rat skin. Methods： A circular full-thickness skin wound（1cm diameter） was created on the back skin of normal rats and diabetic rats, about 1 cm from the skull base.The diabetic rats were divided into three groups：noninjected group, injected pTracer-CMV group and injected pTracer-CMV-HSP47 group. Periwound skin tissues were examined at 3rd, 5th and 7th day. Immunohistochemical and Western immunoblotting were adopted to detect the expression of HSP47 in the tissues during healing of rat skin wounds. Results： Expressions of HSP47 remarkedly increased during healing process in normal rats. Expression of HSP47 during healing in diabetic rats wounds were much weaker. Being injected pTracer-CMV- HSP47, expression of HSP47 in the periwound tissues of diabetic rat increased. Conclusion： After being injected with pTracer-CMV-HSP47, expression of HSP47 increased in diabetic rat wound.
作者 金啸 王佐林
机构地区 同济大学附属口腔医学院口腔颌面外科
出处 《口腔颌面外科杂志》 CAS 2007年第1期11-16,共6页 Journal of Oral and Maxillofacial Surgery
基金 国家自然科学基金项目(30471905) 上海市科委(034119840 044119648 05PJ14097)
关键词 热休克蛋白47 质粒 糖尿病 创口愈合 皮肤 heat shock protein 47 plasmid diabetes wound healing skin
分类号 R587.2 [医药卫生—内分泌]
  • 相关文献

参考文献16

  • 1Oguro A,Sakurai T,Otawa M,et al.The content of heat shock protein 47 (HSP47),a collagen-specific stress protein,changes with gravitational conditions in skeletal muscle[J].Biol Sci Space,2003,17(3):206-207.
  • 2Sauk JJ,Nikitakis N,Siavash H,et al.Hsp47 a novel collagen binding serpin chaperone,autoantigen and therapeutic target[J].Front Biosci,2005,1(10):107-118.
  • 3Ishii H,Mukae H,Kakugawa T,et al.Increased expression of collagen-binding heat shock protein 47 in murine bleomycin-induced pneumopathy[J].Am J Physiol Lung Cell Mol Physiol,2003,285(4):957-963.
  • 4Halper J,Griffin A,Kisaalita WS,et al.In vitro culture decreases the expression of TGF(beta),Hsp47 and type Iprocollagen and increases the expression of CTGF in avian tendon explants[J].J Musculoskelet Neuronal Interact,2005,5(1):53-63.
  • 5程杰,王佐林.大鼠热休克蛋白47真核表达载体的体外构建[J].口腔颌面外科杂志,2007,17(1):6-10. 被引量：2
  • 6Obayashi K,Akamatsu H,Okano Y,et al.Exogenous nitric oxide enhances the synthesis of type Ⅰ collagen and heat shock protein 47 by normal human dermal fibroblasts[J].J Dermatol Sci,2006,41(2):121-126.
  • 7Kakugawa T,Mukae H,Hayashi T,et al.Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia[J].Respir Res,2005,14(6):57-62.
  • 8Koide T,Asada S,Takahara Y,et al.Specific recognition of the collagen triple helix by chaperone HSP47:minimal structural requirement and spatial molecular orientation[J].J Biol Chem,2006,281(6):3432-3438.
  • 9Wang ZL,Inokuchi T,Nemoto TK,et al.Antisense oligonucleotide against collagen-specific molecular chaperone 47-kDa heat shock protein suppresses sear formation in rat wounds[J].Plast Reconstr Surg,2003,111(6):1980-1987.
  • 10Ohba S.Wang ZL,Baba TT,et al.Antisense oligonucleotide against 47-kDa heat shock protein (HSP47) inhibits wound-induced enhancement of collagen production.Arch Oral Biol,2003,48(9):627-633.

二级参考文献14

  • 1王佐林,邱蔚六,井口次夫,根本孝幸.HSP47在胎鼠皮肤创口愈合中的表达[J].口腔颌面外科杂志,2004,14(2):95-99. 被引量：2
  • 2王佐林,张萍,陈万涛,张志愿,邱蔚六.HSP47和TGFβ1在大鼠皮肤瘢痕形成中的作用[J].口腔颌面外科杂志,2004,14(4):293-298. 被引量：5
  • 3Dafforn TR,Della M,Miller AD.The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis[J].J Biol Chem,2001,276(52):49310-49319.
  • 4Koide T,Asada S,Takahara Y,et al.Specific recognition of the collagen triple helix by chaperone HSP47:minimal structural requirement and spatial molecular orientation[J].J Biol Chem,2006,281(6):3432-3428.
  • 5Koide T,Nishikawa Y,Asada S,et al.Specific recognition of the collagen triple helix by chaperone HSP47.Ⅱ.The HSP47-binding structural motif in collagens and related proteins[J].J Biol Chem,2006,281(16):11177-11185.
  • 6Wang ZL,Inokuchi T,Ikeda H,et al.Collagen-binding heat shock protein HSP47 expression during healing of fetal skin wounds[J].Int J Oral Maxillofac Surg,2002,31(2):179-184.
  • 7Razzaque MS,Taguchi T.The possible role of colligin/HSP47,a collagen-binding protein,in the pathogenesis of human and experimental fibrotic diseases[J].Histol Histopathol,1999,14(4):1199-1212.
  • 8Kakugawa T,Mukae H,Hayashi T,et al.Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia[J].Respir Res,2005,6(1):57-60.
  • 9Ohashi S,Abe H,Takahashi T,et al.Advanced glycation end products increase collagen-specific chaperone protein in mouse diabetic nephropathy[J].J Biol Chem,2004,279(19):19816-19823.
  • 10Hirai K,Kikuchi S,Kurita A,et al.Immunohistochemical distribution of heat shock protein 47 (HSP47) in scirrhous carcinoma of the stomach[J].Anticancer Res,2006,26(1A):71-78.

共引文献1

同被引文献18

  • 1程杰,王佐林.大鼠热休克蛋白47真核表达载体的体外构建[J].口腔颌面外科杂志,2007,17(1):6-10. 被引量：2
  • 2Falanga V. Wound healing and its impairment in the diabetic foot [J]. Lancet, 2005, 366(9498):1736-1743.
  • 3Brown DL, Kane CD. Chemausek SD, et al. Differential expression and localization of insulin-like growth factors 1 and II in cutaneous wounds of diabetic and nondiabetie mice [J]. Am J Pathol,1997, 151(3):715-724.
  • 4Beer HD, Longaker MT, Wemer S, et al. Reduced expression of PDGF and PDGF receptors during impaired wound healing [J]. J Invest Dermatol, 1997. 109(2): 132-138.
  • 5Schaffer MR, Tantiy U, Efron PA. et al. Diabetes-impaired healing and reduced wound nitric oxide synthesis: a possible pathophysiologic correlation [J]. Surgery.1997.121(5): 513-519.
  • 6Bitar MS. Labbad ZN. Transforming growth factor-beta and insulin-like growth factor-I in relation to diabetes-induced impairment of wound healing [J]. J Surg Res. 1996. 61(1): 113-119.
  • 7Nagata K. HSP47 as a collagen-specific molecular chaperone: function and expression in normal mouse development [J]. Semin Cell Dev Biol, 2003, 14(5):275-282.
  • 8Rocnik EF, van der Veer E, Cao H, et al. Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells [J]. J Biol Chem, 2002,277(41):38571-28578.
  • 9Wang ZL, Inokuchi T. Nemoto TK, et al. Antisense oligonucleotide against collagen-specific molecular chap- erone 47-kDa heat shock protein suppresses scar formation in rat wounds [J]. Plast Reconstr Surg, 2003;111 (6): 1980-1987.
  • 10Keagle JN, Welch W J, Young DM. Expression of heat shock proteins in a linear rodent wound[J]. Wound Repair Regen, 2001,9(5):378-385.

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口腔颌面外科杂志
2007年 第1期

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