摘要
以天然野生植物火棘果为原料分离提取精氨酸,考察了树脂类型、吸附方式、温度、pH值、洗脱液、脱色及精制方法等因素对提取精氨酸的影响,并对产物进行了分析鉴定。结果表明,在20℃、pH值7-8的条件下,选用D001型大孔阳离子交换树脂静态吸附,40 min即达到饱和;以3 mol.L-1氨水为洗脱液,流速控制在40 mL.h-1,精氨酸的洗脱率达到90%以上;采用711型阴离子树脂进行脱色,D001型大孔阳离子交换树脂动态精制,精氨酸的提取率达到95.83%。
Arginine was extracted from the natural wild plant Pyracantha. The resin type, absorption way, temperature, pH value, eluent, decolorization and purification were investigated. The result indicated： Using D001 macropore cation exchange resin at static adsorption, when the temperature was 20℃ and the pH value was between 7 and 8, the saturation could be achieved in 40min ; The arginine eluation rate achieved above 90% when the eluent was 3 mol·L^-1 ammonia and the velocity of flow was 40mL·h^-1 ; The product was decolored by 711 anion resin and purified dynamically by D001 macropore cation exchange resin, and the extraction rate was upto 95.83 %.
出处
《氨基酸和生物资源》
CAS
2007年第1期47-50,共4页
Amino Acids & Biotic Resources
