摘要
本文报告了江苏省１９９４～１９９５年急性弛缓性麻痹（ＡＦＰ）病例的病原学监测结果。共检测３５８例粪便标本，分离到病毒５０株，阳性率为１４．０％。其中检出脊髓灰质炎（脊灰）病毒１７株，阳性率为４．７％。对１９９４、１９９５年分离到脊灰病毒的１３例病人的传代标本，用ＰＣＲ法鉴定型内特征均为疫苗株。初步表明，脊灰散在发病，型别已由１９８９、１９９０年流行时期Ⅰ型为主转向Ⅱ、Ⅲ型，且分离的脊灰病毒均为疫苗株。
his paper reported the surveilianee results of AFP cases and its etiological analysis in Jiangsuprovince in 1994-1995.Totally,358 AFP fecal specimens wrre tested and 50 virus isolathe were de-rived,the virus positive isolation rdte was 14.0%. Among them1 2 were polio viruses and 33 theenteroviruses.Through microneutralization test,2 polioⅠviruses,10 polio Ⅱvirused,2 polio Ⅲviruses,2 polio Ⅰ+ Ⅱ mixed virused and 1 polioⅠ+Ⅲmixed viruses were identified.This asults indicated that virus types of sporadic polio cases in 1994-1995 converted into type Ⅱand type Ⅲ from typeⅠwhich had been dominant in 1 989 and 1 990.Futher,by using RFLP-PCR technique to analyze intrdtypic differentiation of 1 3 isolated polio virus strains,it proved they were all polio vaccine virus strdins.
出处
《中国计划免疫》
1996年第5期218-220,共3页
Chinese Journal of Vaccines and Immunization
关键词
弛缓性麻痹
脊髓灰质炎
病原学
监测
Acute flaccid pardlysis(AFP),Poliomyelitis,Virus isolation and identification,PCR
