摘要
目的:建立人口腔扁平苔藓(OLP)及正常黏膜组织的双向凝胶电泳图谱,分析其差异表达的蛋白质,筛选分子标记物。方法:采用固相pH梯度(immobilized pH gradient,IPG)双向凝胶电泳(two-dimensional gel electrophoresis,2-DE)分离OLP及正常黏膜组织的总蛋白质,银染显色,Imagemaster图像分析软件分析差异蛋白质点。结果:①人口腔扁平苔藓及正常黏膜组织凝胶的平均蛋白质点数分别为1576±67和1608±73,凝胶图谱分辨率、重复性较好。②通过比较OLP与正常黏膜组织的双向凝胶电泳图谱,差异表达蛋白质点数为13个,其中7个点在口腔扁平苔藓中为高表达,6个点在OLP中为低表达。结论:本研究建立了分辨率高且重复性较好的OLP及正常黏膜组织的双向凝胶电泳图谱,发现存在一些差异表达的蛋白质,为进一步筛选OLP特异性的分子标志物奠定了基础。
Objective ： To establish the two-dimensional Gel electrophoresis profiles with high resolution and reproducibility from human oral lichen planus and paired normal oral mucosa tissues , and identify the differential expression proteins. Method ： The total proteins of oral lichen planus and paired normal oral mucosa tissues were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis （2-DE）.After AgNO3 staining, the differential expression proteins were analyzed by using ImageMaster image analysis software. Result ： （1）The well-resolved, reproducible 2-DE patterns of oral lichen planus and normal oral mucosa tissues were obtained. The results showed that average spots were 1576±67, 1608±73 in oral lichen planus and normal oral mucosa tissues respectively. （2） The 13 differential protein spots were identified by Image Master image analysis software. There were 7 protein spots in oral lichen planus were higher expression than that in normal oral mucosa. 6 protein spots in oral lichen planus were lower expression than that in normal oral mucosa. Conclusion ： In this study, the well-resolved reproducible 2-DE patterns of oral lichen planus and normal oral mucosa tissues were established and some differential proteins were characterized.These data will be further used to screen special biomarkers of oral lichen planus.
出处
《临床口腔医学杂志》
2007年第2期117-120,共4页
Journal of Clinical Stomatology
基金
南京医学发展基金项目资助
关键词
口腔扁平苔藓
双向凝胶电泳
蛋白质组
oral lichen planus
two-dimensional gel electrophoresis
proteome
