摘要
[目的]比较液相与固相蛋白芯片检测多肿瘤标记物的临床应用效果,并探讨液相蛋白芯片应用于临床检验的可行性。[方法]分别用液相与固相蛋白芯片方法检测45例临床送检样本的AFP、CA125、CA242及CEA,对两种方法的操作步骤、有效检测范围、参考临界值及检测结果进行比较。[结果]与固相蛋白芯片相比,液相蛋白芯片检测多肿瘤标记物的操作至少节省1h,标本用量节省80μl,线性范围至少增大1倍;两种方法检测AFP、CA125、CA242及CEA的参考临界值相同,阴阳性判断结果的符合率分别为97.78%(44/45)、86.67%(39/45)、88.89%(40/45)、86.67%(39/45)。统计学分析表明两种方法检测的阴阳性结果无明显差异。[结论]液相蛋白芯片检测多肿瘤标志物具有操作简便、标本用量少、线性范围广、准确度高的优点,可推广应用于临床检验。
[Objective] To compare the results of clinical application of liquid and solid protein chip technology, in detecting multiple tumor markers, and discuss the feasibility of liquid protein chip in clinical practice. [ Methods] AFP, CA125, CA242 and CEA in 45 clinical cases were detected by liquid and solid protein chip technology respectively. The manipulation, the linear range, the referenced critical values and the results were compared. [Results] Comparing with the solid protein chip, the operational time reduced by one hour, the sample amount reduced by 80μl, and the linear range increased at least one time by the liquid pmtein chip in detecting multiple tumor markers. The referenced critical values of AFP, CA125, CA242 and CEA were all the same by the two assaies. The accordance rate were 97.78% （44/45）, 86.67% （39/45）, 88.89% （40/45）, 86.67% （39/45） respectively. Statistical analysis indicated that the results detected by the two assaies were not different. [Conclusions] The liquid protein chip has the merit of simpler operation, snlaller sample amount, wider linear range and higher accuracy in detecting multiple tumor markers. It can be used in clinical practice widely.
出处
《现代预防医学》
CAS
北大核心
2007年第6期1050-1051,1054,共3页
Modern Preventive Medicine
