摘要
采用永久双侧椎动脉热凝烧灼术,次日行双侧暂时夹闭阻断颈总动脉20 min再灌48 h法制备pullinsin-4vo大鼠模型.利用Morris水迷宫检测模型大鼠空间学习记忆能力,用组织化学技术显示模型大鼠脑组织病理改变.造模前模型组水迷宫定位航行试验的逃避潜伏期与正常组和假手术组差别无显著性;再灌48 h后模型大鼠的逃避潜伏期及游泳路径长度显著长于正常组和假手术组,P值均小于0.05;免疫组织化学观察显示模型大鼠海马组织存在明显的缺血坏死病灶,海马各区神经细胞结构排列紊乱、稀疏,细胞数明显较少.研究表明经改良后的血管性痴呆大鼠模型是进行血管性痴呆病理机制及其药物疗效评价的理想动物模型.
Apply permanent arteria vertebralis heat- blocked and temporary carotid artery occlusion （20 rain） reperfusion （48 h） on a new Pullisin - 4VO animal model. Morris Maze test for the ability of space learning and memory, Immunohistochemical technique were used to present the pathology change of brain tissue. The escaping period and the route of model is no difference before model making, and significantly longer than the normal and sham operated （ P 〈 0.05）. The necrosis of hippocampus neutrons occurred obviously after ischemia for 20 min and reperfusion 48h. the up grade animal model is suitable to estimate the pathomechanism and drug curative effect in vascular dementia.
出处
《韶关学院学报》
2006年第12期91-93,共3页
Journal of Shaoguan University
关键词
血管性痴呆
动物模型
水迷宫
学习记忆
vascular dementia
animal models
morris water mazz
learning and memory
