摘要
为了研究GA3和ABA对种子萌发过程中产生的β-甘露聚糖酶活性的影响,以水稻种子作为研究材料,测定了GA3和ABA存在情况下的β-甘露聚糖酶活性,结果表明:GA3和ABA不仅对水稻种子萌发有明显的促进和抑制作用,对种子萌发过程中β-甘露聚糖酶活性也有显著的影响。50μMGA3可使β-甘露聚糖酶活性出现的时间从48h提早到吸水36h,并且显著提高酶的活性。100μMABA在强烈抑制种子正常萌发的同时也抑制了β-甘露聚糖酶活性。但是50μMGA3可恢复ABA对β-甘露聚糖酶活性的抑制作用。去胚的半粒水稻种子在水中吸涨时检测不到β-甘露聚糖酶的活性,但在水中加入50μMGA3后,可恢复去胚半粒种子产生酶活性的能力。因此,推测水稻种子萌发时β-甘露聚糖酶活性的产生可能是由胚中而来的某种物质所诱导,而这种物质很可能就是GA3。
In order to investigate the effect of phytohormone on the activities of endo-β -mannanase which was produced during and following seed germination, we took the rice （ Oryza sativa L. cv. Taizhong 65, a japonica subspecies） seeds as material. The results showed that GA3 and ABA could promote and inhibit germination of rice seeds, and could also remarkably influence the expression of endo-β-mannanase activity. The first detection of endo-β -mannanase activity was brought forward from 48 h to 36 h from the start of imbibition and activity was increased dramatically by 50 uM GA3, whereas germination percent and endo-β -mannanase activity were inhibited by 100 uM ABA, and 50 uM GA3 could suppress the effect of ABA to some extent. By cutting rice seeds into emhryoless half-grains, it was shown that embryo was required for the synthesis of endo-β -mannanase in the aleurone layer and this role of embryo could be replaced by exogenous GA3.
出处
《种子》
CSCD
北大核心
2007年第2期6-9,共4页
Seed
基金
国家自然科学基金(30370913)。
