摘要
在单因素预试验的基础上,采用正交试验设计,筛选出了植物组织培养中红金银花外植体最佳灭菌方法,总结出外植体的类型、酒精浸泡时间、升汞质量分数、升汞浸泡时间等对红金银花外植体的污染程度及成活率均有显著影响。采用L8(4×24)正交试验表明,对红金银花外植体先用75%的酒精消毒30 s,再配合0.2%的升汞溶液处理5 m in,能达到理想的灭菌效果。
The sterilization method of the explants of Lonicera japonica var. chinensis was screened out with orthogonal design and single factor experiment. The results were as follows：such factors as explant type, concentration of HgCl2 , sterilization time in ethanol and HgCl2 had significant effects on the contaminative rate and the survival of the explants. The results of orthogonal experiment 1,8 （4 × 2^4） showed that it was optimal for the explants of Lonicera japonica var. chinensis to be treated with 75% ethanol for 30 s and subsequently 0. 2% HgCl2 for 5 min.
出处
《江苏林业科技》
2007年第1期23-25,共3页
Journal of Jiangsu Forestry Science & Technology
关键词
红金银花
组织培养
外植体
灭菌
Lonicera japonica var. chinensis
Tissue culture
Explant
Sterilization
