摘要
用真核表达的ＨＰＶ１６Ｌ１／Ｌ２蛋白作为抗原，经免疫、融合、选择性培养、克隆化等过程，我们建立了两株抗ＨＰＶ１６Ｌ１／Ｌ２蛋白的杂交瘤细胞株（另有数株仍在建株中）。免疫斑点法检测证明，它们产生的抗体，只与ＨＰＶ１６Ｌ１／Ｌ２蛋白起反应，而不与ＨＰＶ１６Ｅ６、Ｅ７蛋白、人血清蛋白和牛血清蛋白起反应。另外，免疫组化试验证明，这种抗体可应用于临床ＨＰＶ１６感染的检验，具有敏感、特异、准确、快捷、经济的优点。
Two hybridoma cell lines secreting McAbs: against HPV16 virus-like particles, which comprises eukaryotic expression system derived L1/L2 capsid proteins, were established. The antibodiesthey secreted only react with HPV16 virus-like Particles while have no reaction with HPV16 E6/E7protein, human serum albumin or bovine serum albumin.It was proved immunohistochemically thatthese antibodies could be sensitively and specifically used in clinical detection of HPV16 infection.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第1期32-34,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
美国中华医学基金
关键词
人乳头瘤病毒
L1/L2蛋白
单克隆抗体
human Papillomavirus type 16(HPV16)
virus-like particles
monoclonal antibody
