摘要
参照立氏立克次体分子量为１２×１０＾４蛋白基因序列合成引物，分两段扩增日本立克次体的１２×１０＾４蛋白基因，扩增ＤＮＡ的长度分别为２．５和２．６ｋｂ。含有启动子区的２．６ｋｂ片段在ｐＵＣ１９质粒中不稳定。从这段ＤＮＡ删去启动子及８５个氨基酸编码子区的扩增产物可被克隆。
The gene encoding 120 x 103 outer membrane protein (ompB) of Rickettsia japonica was PCR-amplified by using the primers synthesized with reference to the ompB gene sequence of R.rickettsii. Two DNA fragments with molecular size of approximate 2. 5 and 2. 6kb were produced. The 2. 6kb fragment containing rickettsial promotor region of the gene was found to be unstable in pU C 1 9 plasmid , but an amplified product removing the promotor and coding region of 95 amino acids .from this segment could be cloned into the plasmid. Thus the recombinant plasmid was served as a vector to clone the 2. 5kb fra8ment containing the stop codon of the gene. Sequence analysis revealed an ORF of 4956 nucleotides encoding 1 652 putative amino acids. Genetic homology of the gene between R. japonica and R. rickettsii is 95. 5% , suggesting that the ompB may be a potential candidate for use as a subunit vaccine against SFG rickettsial infection .
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第3期220-226,共7页
Chinese Journal of Microbiology and Immunology
