摘要
本实验以个旧云南锡业公司某矿坑下作业工人和某冶炼厂尿砷,尿铅超标工人为检查对象,初步探讨了污染与人类染色体损伤的关系,结果是使以上受检对象的外周淋巴细胞的染色单体断裂率、裂隙率、畸变细胞率和姐妹染色单体交换率都明显增高,与对照组相比差异显著。尿铅和尿砷超标工人外周淋巴细胞的染色体畸变和姐妹染色单体交换率分别与对照组相比,经统计学处理差异显著(P<0.05),且随着砷、铅在体内蓄积量的增加而有上升的趋势,表明砷、铅污染是引起人类染色体损伤的一个因素。
The chromosomal aberrations and sister chromatid exchanges of the peripheral lymphocytes from the workers in different polluted environments were observed, and the results are as follows. 1. Average frequencies of the cells with chromosomal aberrations and breakages were 2.47% and 2.53% for pit workers respectively, 3.22% and 3.29% for smelters, 1.00% and 1.10% for up-pit workers, and 0.4% and 0.5% for non-exposed workers.
2. Average frequencies of SCEs were 4.25±0.13 for pit workers, 3.49±0.10 for up-pit workers, 3.26±0.09 for smelters, and 2.4610.09 for non-exposed workers. 3. In three groupcs with different urine arsenic level, average frequencies of SCEs were 5.35±0.31, 5.76±.24, and 5.60±0.19, respectively, and average frequencies of chromosomal aberrations and breakages were 1.75%, 2.16%, 1.90% and 1.75%, 2.16%, 2.00%, compared with 0.6% for each control group.
4. In four groups with different urine lead, average frequencies of SCEs were 5.07±0.1, 5.82±0.21, and 5.96±0.09 respectively, and average frequencies of the cells with chrosomal aberrations and breakages were 2.53%, 2.18%,2.59%,2.42% and 2.58%,2.36%, 2.42% and 2.66%, compared with 0.6% for each control group.
The difference between the exposed groups and each control groups are statistically significant.
关键词
砷
铅
淋巴细胞
染色体畸变
SCE
arsenic,lead, SCEs, chromosomal aberrations, lymphocyte
