摘要
在HEPES缓冲,含15—20％胎牛血清,100单位青霉素/ml,100微克链霉素/ml的RPMI-1640培养基中,用人胚肺成纤维细胞(KMB)和新生鼠肾成纤维细胞型细胞(ZUKF)作支持细胞,成功地使伊氏锥虫(Trypanosoma evansi)正常动基体株(kinetoplastic strain)和异常动基体株(dyskinetoplastic strain)在37℃含5％ CO_2的二氧化碳培养箱中连续培养达60天以上,并保持其对小鼠的高度感染力和致病力。这是迄今为止首次利用来源于人的细胞作支持细胞连续培养家畜非洲锥虫血液型成功的报告。实验结果表明伊氏锥虫体外培养成功与否对提供支持细胞的宿主是否有感染性无必然的联系。此外,利用Baltz氏等建立的无支持细胞培养系统,我们亦成功地使上述两伊氏锥虫株在体外连续繁殖,并保持对小鼠的高度感染力和致病力。
Kinetoplastic and dyskinetoplastic strains of T. evansi were successfully cultured at 37℃ cn a feeder layer of fibroblasts from human embryo lung (KMB) or from newborn mice kidney (ZUKF) in Hepes-buffered RPMI-1640, supplemented with 15-20% heat-inactivated fetal bovine serum (FBS) plus 100 U penicillin/ml and 100 μg/ml streptomycin/ml. This is the first report of using human cell as supporting cell to culture the trypanosome of domestic animal. The parasites of these two strains retained their infectivity to mice after culturing in vitro for more than 60 days. The growth curves of these two strains of T. evansi in KMB cell and in ZUKF cell showed no obvious difference. Such results indicated that the donors of suitable supporting cells for culture of T. evansi did not necessarily correspond to the susceptible hosts of this parasite. Meanwhile, the two strains of T. evansi were also successfully cultured in the Baltz's semi-defined medium system. These parasites kept their infectivity to mice after cultivation in Baltz's culture system in vitro for more than 60 days as well. The cultured trypano-somes of these two strains showed similar characteristics in morphology, variant antigenic surface coat, ultrastructure and infectivity as those in in vivo parasites.
出处
《动物学报》
SCIE
CAS
CSCD
1990年第3期299-305,共7页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
中山大学科学基金
关键词
伊氏锥虫
KMB
ZUKF
体外培养
Trypanosoma evansi, human embryo lung fibroblast, newborn mice kidney fibroblast-like cell, in vitro.
