摘要
目的探讨痛性椎间盘神经纤维内生长的原因。方法以正常、内破裂及突出椎间盘中提取的聚集蛋白聚糖与培养基一起培养人骨髓神经母细胞瘤（SH-SY5Y），磷酸盐缓冲溶液（PBS）作对照处理，检测比较神经细胞轴突发生率及轴突长度。结果浓度为100mg／L时，内破裂及突出椎间盘髓核和纤维环聚集蛋白聚糖处理组中神经细胞轴突发生率及其长度[（44．8±2．8）％／（41．8±2．0）um和（34．2±2．9）％／（37．2±3．1）um；（56．0±1．5）％／（51．8±2．2）um和（48．8±1．5）％／（48．2±2．2）um]较正常对照组[（34．2±2．6）％／（29．0±2．5）um和（23．4±2．6）％／（26．2±2．2）um]明显增加（P〈0．05）；浓度为10mg／L或1000mg／L时，3者之间差异无统计学意义（P〉0．05），但均具有类似的上升趋势。结论痛性椎间盘中聚集蛋白聚糖对神经生长抑制能力减弱，有利于椎间盘源性下腰痛的产生。
Objective To explore the cause of ingrowth of nerves into disc tissue which induced discogenic low back pain. Methods Disc aggrecan was extracted from human normal, internal disc disruptive （IDD） and lumbar intervertebral discs protrusion （LIDP） intervertebral discs and incorporated into tissue culture substrata,respectively, for the culture of the human neuronal cell line, SH-SYSY. The percentage of SH-SYSY cells extending neurites and the mean length of the neuritis, as the effects on nerve growth of different aggrcan, were determined and compared. PBS-pretreated cultures were used as controls. Results At concentration of 100 mg/L aggrecan, the percentage of SH-SYSY cells extending neuritis was higher and the mean length of the neuritis longer in IDD and LIDP nucleus pulposus and annular fibrosus aggrecan treated cultures [ （44.8 ± 2.8） % / （41.8 ± 2.0） um and （ 34.2 ± 2.9） % / （ 37.2 ± 3.1 ） um; （56.0±1.5）%/（51.8±2.2） um and （48.8±1.5）%/（48.2±2.2） um] than those in normal disc aggrecan-treated cultures [ （ 34.2 ± 2.6） % / （ 29.0 ± 2.5 ） um and （ 23.4 ± 2.6） % / （ 26.2 ± 2.2） um ]. Although there was no significant difference in both the proportions of neurite initiation and neurite lengths on the coverslips pretreated with nucleus pulposus and annular fibrosus disc aggrecan from different samples at concentrations of 10 mg/L and 1000 mg/L, there was a trend towards increased proportions of cells with neuritis and neurite length in the IDD or LIDP vs normal and in the LIDP vs IDD. Conclusion The capacity of disc aggrecan to inhibit nerve growth was reduced in intervertebral discs of IDD and LIDP, which may play a direct role in the associated ingrowth of nerves.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第3期319-320,共2页
Chinese Journal of Experimental Surgery
关键词
椎间盘
神经
生长
Intervertebral disc
Nerve
Growth
