摘要
目的:分析体表高频超声检测家兔血管内球囊成形术后再狭窄程度与组织病理学分析的相关性。评估体表超声检测的可行性、可靠性及应用价值。方法:实验于2002-03/2003-12在北京中医药大学中医内科学重点学科实验室完成。①日本大耳白兔26只,随机分为正常组10只、假手术组6只、模型组10只。②模型组电刺激兔颈总动脉,电流1.2mA,刺激12～15min,术后第2天喂饲高脂饲料共8周,假手术组仅剥离颈总动脉,不做电刺激,喂高脂饲料,正常组不施加任何干预因素。③模型组和假手术组根据B超选择颈总动脉有斑块或血流明显改变者行球囊血管内成形术。分别于电刺激后8周、血管成形术后4周行超声检查动脉内径和动脉内膜厚度。④所有动物于血管成形术后4周处死取材,进行病理学半定量分析,并与超声测量结果进行相关分析。结果:进入结果分析数量24只,正常组中途死亡1只,原因为牙齿畸型影响进食。模型组1只因电刺激8周时超声评价颈动脉未形成斑块及血流无明显改变而剔出实验。①超声检测电刺激8周时正常组内膜厚为(0.028±0.004)cm,模型组管壁明显增厚(0.043±0.014)cm,差异有显著性(P<0.05),至血管成形术后膜厚增加更明显(0.064±0.002)cm,内径稍有扩大,但差异不显著。②超声检测模型组颈动脉内径与膜厚的测量结果与病理学测量结果呈正相关关系(OR=0.361,P<0.05;OR=0.526,P<0.01),病理狭窄率与超声是否检测到斑块呈正相关关系(OR=0.796,P<0.01)。结论:体表高频超声在评价家兔颈动脉狭窄诊断中有一定应用价值,与病理学半定量分析结果相关性良好。
AIM： To investigate the correlation between the degree of restenosis after percutaneous transluminal angioplasty （PTA） by high-frequency ultrasound and pathologic analysis, and evaluate the feasibility, reliability and practical value of high-frequency ultrasound.
METHODS： The experiment was conducted in the Key Laboratory of Internal Medicine, Beijing University of Traditional Chinese Medicine from March 2002 to December 2003. （1） Twenty-six Japan flap-eared white rabbits were divided randomly into the normal control group （n =10）, sham-operation group （n =6） and model group （n =10）. （2） Animals in the model group were stimulated of the carotid arteries by electric current for 12-15 minutes at 1.2 mA, and rabbits were fed with high-fat diet for 8 weeks since the second day post-operation, while animals in the sham-operation group were only separated of the carotid artery without electric stimulation and fed with high-fat diet. Rabbits in the normal control group received no intervention. （3）According to B ultrasound, animals with blot in the common carotid artery and obvious changes in the model group and sham-operation group were administrated with PTA, and the internal diameter of artery and the thickness of tunica intima were inspected by ultrasound at 8 weeks after electric stimulation and 4 weeks after PTA respectively. （4） All animals were executed at 4 weeks after the PTA for pathologic semiquantitative analysis, and correlative analysis was perfored with the result measured by ultrasound.
RESULTS： Twenty-four animals were involved in the analysis of results, and one rabbit of the normal control group died in the course for unable to eat due to dysplastic teeth. One rabbit in the model group was excluded for that there was no blot in the carotid artery or changes in blood current. （1） The thickness of tunica intima in the normal control group at the 8th week of electric stimulation was （0.028±0.004） cm, while the vessel wall of the model group was obviously thickened to （0.043±0.014） cm, and the difference was remarkable （P 〈 0.05）. The tunica intima was thickened more remarkable after PTA （0.064±0.002） cm, and the internal diameter was enlarged a little without significant difference. （2） Ultrasound detection showed that the internal diameter of carotid artery and thickness of tunica intima and tunica media were in positive linear correlation pathologically （OR =0.361, P 〈 0.05;OR =0.526, P 〈 0.01）. The B-ultrasomic blot and pathologic straitness have significant correlation （OR =0.796, P 〈 0.01 ）.
CONCLUSION： High-frequency ultrasound is a valuable in the diagnosis of straitness of rabbits＇ carotid, and it has a good correlation with pathology.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第9期1624-1627,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家中医药管理局科技项目(2000-J-P-21)~~
