摘要
为了探讨芦苇在高浓度畜禽废水污染胁迫下遗传特性的变化,将人工湿地试验池中生长的,受高浓度畜禽废水长期污染胁迫的和未受到污染的供试芦苇材料各20个单株幼芽分别混合提取DNA,采用RAPD分子标记技术在160个随机引物中进行筛选,找到3个能够扩增出差异且重复性较好的RAPD引物:S74、S516和S14。结果表明,在高浓度畜禽废水长期污染胁迫下,芦苇在DNA水平上产生了变异,其变异产生的特异性扩增片段可能与芦苇抗污染基因紧密连锁,芦苇在高浓度畜禽废水污染胁迫下的抗污染旺盛生长特性也可能与这种变异有关。同时,本研究结果能为在分子水平上进一步揭示人工湿地植物的强去污能力机理和抗污染特性提供参考依据。
The plumules of Phragmites australis growing in the constructed wetland test pool with high-concentration livestock wastewater and clean water were collected, and the total DNA was extracted for RAPD analysis to explore the genetic variation of Phragmites australis under contamination stress of high-concentration livestock wastewater. RAPD PCR amplification using 160 primers showed that there were three primers, S74, S516 and S14, displayed repeatable and different RAPD fingerprinting patterns between the materials existed. The results also indicated that Phragmites australis was mutated at molecular level under the stress of high-concentration livestock wastewater. The specific RAPD fragments caused by this mutation might be closely linked with the anti-polluted gene of Phragmites australis. The excellent growth performance of the plants under high-concentration livestock wastewater could be relevant to this gene mutation. This work will provide the basis for further research on revealing the molecular mechanism of strong anti-polluted capability of constructed wetland growing plants.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第1期92-96,共5页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(30570062)
关键词
人工湿地
芦苇
RAPD
畜禽废水
污染胁迫
constructed wetland
Phragmites australis
RAPD
livestock wastewater
, contamination stress
