摘要
串珠藻目(Batrachospermales)是淡水红藻中最主要的类群。近年来,应用DNA序列分析探讨串珠藻目的系统发育,并与传统的形态学和生态学特征相结合,为串珠藻目系统学研究拓展了新的思路。本文回顾了串珠藻目的建立及其所含类群的研究历史,归纳了目前在串珠藻目系统发育与进化研究中常用的分子标记方法,其中包括核基因组的18S rDNA、26S rDNA和ITS序列,叶绿体基因组的rbcL序列,线粒体基因组的cox2-3序列,以及新兴的ISSR技术,并对各种分子标记的特点及适用范围做了评述。结果表明,ITS序列多适用于种群分化及相近种间遗传分析,ISSR标记适用于种下分类群间及同一种群不同个体间基因多态性分析,cox2-3序列在一定程度上也可用于同一种群不同个体间的基因多态性分析,而18S rDNA与rbcL序列既可用于种间关系分析,又可用于更高水平分析的构建系统树。这些分子标记已被证明在研究串珠藻目系统地理、物种起源和散布机制方面有着广泛的应用前景。同时,本文对串珠藻目分子系统学研究的最新进展也进行了概述,并对今后的研究方向做了展望。
Batrachospermales are the largest group of freshwater red algae. Recently, DNA sequence analysis has been widely used in molecular systematics of Batrachospermales and offers a new path for phylogenetics study combined with traditionally morphological and ecological methods. This paper reviews the foundation of Batrachospermales and the groups included, then summarizes the molecular markers commonly applied for the study of the phylogeny and evolution of Batrachospermales. The algae show 18S rDNA, 26S rDNA and an internal transcribed spacer （ITS） region in the nuclear genome, an rbcL sequence in chloroplast DNA, and a cox2-3 spacer in mitochondrial DNA. Also discussed is the new technique of inter simple sequence repeat （ISSR） and the expansion of the applicable range of each molecular marker. The ITS region is mainly used for population differentiation and genetic analysis among closely related species, and ISSR is applicable for studying genetic diversity of interpopulations and individual variation. As well, the cox2-3 spacer is sometimes used for investigating genetic diversity of individual variation, whereas the rbcL sequence and 18S rDNA could be used for molecular studies at interspecific and higher taxonomic levels. These markers have been shown in phylogeography, dispersal mechanisms and the origin of species. The authors introduce the latest progress in this area and discuss the prospects for future research.
出处
《植物学通报》
CSCD
北大核心
2007年第2期141-146,共6页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.30470118和30270119)
关键词
串珠藻目
分子系统学
序列分析
Batrachospermales, molecular systematics, sequence analysis
