摘要
目的研究抑癌基因PTEN(phosphatase and tensinhomolog detected on chromosometen)编码蛋白在正常结肠黏膜、结肠腺瘤、癌旁结肠黏膜及结直肠癌组织中的表达特征。探讨结直肠癌中PTEN表达的临床病理学意义。方法采用免疫组织化学(S-P法)检测110例结直肠癌、13例癌旁黏膜、17例结肠腺瘤、8例正常结肠黏膜中PTEN的表达,分析其与结直肠癌临床病理学关系。结果PTEN蛋白在结直肠癌、癌旁黏膜、结肠腺瘤和正常结肠黏膜中的阳性表达率分别为59.1%、76.9%、76.4%和100%,结直肠癌PTEN蛋白的阳性表达率及水平明显低于非癌组,P<0.01。细胞分化较差、有淋巴结转移、Duke分期较晚的结直肠癌PTEN蛋白阳性率表达低,P<0.05。结论PTEN蛋白的表达在大肠癌中呈减少或缺失,并与大肠癌临床病理学特性有关,其表达水平可作为判定结直肠癌临床病理学特性的标志。
OBJECTIVE： To detect the expression of tumor supprecsor PTEN protein in human benign or malignant lesions of the large intestine and investigate its clinical significance in colorectal cancer. METHODS： A total of 110 colorectal cancer tissues, 13 adjacent carcinoma tissues, 17 adenomatous tissues, and 8 normal colorectal tissues were assayed by immunohistochemical S-P meth ods. The relationship between the expression of PTEN protein and clinical pathological features in colorectal cancer was analyzed. RESULTS：The positive rate of PTEN detected in 65（59. 1%） colorectal cancer tissues was much lower （P〈0.05） than that in 13（76.9%） adjacent carcinoma tissues, in 17（76.4 %） adenomatous tissues, and 8（100%） nomal colorectal tissues. The positive rate or the expression level of PTEN in colorectal cancer with lymph node metastases was lower than those in colorectal cancer without lymph node metastases. The level of PTEN was increased with the up regulation of differentiation. The more advanced Duck＇s stage, the lower level of PTEN was. CONCLUSIONS： The expression of PTEN is lost or low, and it has a correlation with clinical pathological features in colorectal cancer. It may be a good biomarker, being helpful to assess clinical pathological features in colorectal cancer.
出处
《中华肿瘤防治杂志》
CAS
2007年第2期126-128,共3页
Chinese Journal of Cancer Prevention and Treatment
