摘要
应用已构建了AFLP遗传图谱的大白菜DH群体，采用多模型QTL作图的方法，通过色差计法、叶色素测定法和人为目测法3种方法7个指标对叶色相关性状进行QTL定位和分析。结果表明，在7个连锁群上共检测到18个QTL，其中色差计法检测到9个QTL，叶色素测定法检测到8个QTL，人为目测法仅检测到1个QTL。另外，估算了单个QTL的贡献率和加性效应，发现各QTL的加性效应各不相等，各位点的贡献率在5．5％～15．6％之间。
A Chinese cabbage （Brassica rapa L. ssp. pekinensis） double-haploid （DH） population with a reported AFLP genetic map was employed to map quantitative trait loci （QTL） related to leaf color by using MAPQTL 4. 0 package. Tristimulus colorimeter, leafy pigment determination and ocular measurement were used to measure the traits related to leaf color. A total of 18 QTL were detected on 7 linkage groups, 9, 8 and 1 QTL were detected by 3 methods, respectively. These QTL individually explained 5. 5% to 15. 6% of the phenotypic variation, and there were unequal gene effects on the traits related to leaf color. These mapped QTL could be used to develop a marker assisted selection program for leaf color in Brassica rapa vegetable varieties.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期99-104,共6页
Acta Horticulturae Sinica
基金
中-荷战略科学联盟项目(‘973’国际合作计划
2004CB720)
中-荷联合园艺作物基因组技术实验室项目(2001CB711102)
农业部蔬菜遗传与生理重点开放实验室项目
