摘要
目的探讨供肝冷保存对肝移植术后肝内胆管微循环的影响。方法实验大鼠随机分为假手术组(SO 组)、供肝冷保存1 h 组(CP 1 h 组)、供肝冷保存24 h 组(CP 24 h 组)。采用重建肝动脉的大鼠肝移植模型。在肝移植术后的不同时间点,观察肝内胆管组织损伤程度;经肝动脉注入微球,光镜下行肝组织汇管区内微球计数;采用间接免疫荧光双染技术检测肝组织汇管区微小血管内皮细胞 eNOS、ET-1和 ICAM-1的表达,并采用原位杂交技术检测其 mRNA 的表达。结果冷保存再灌注可引起肝内胆管结构改变,冷保存时间越长损害程度越重。冷保存再灌注可引起肝组织汇管区内微球数量增加,且时问越长微球数量增加越明显。冷保存再灌注可引起汇管区微小血管内皮细胞eNOS 蛋白及 mRNA 表达水平降低,而 ET-1和 ICAM-1的蛋白及 mRNA 表达水平升高。结论冷保存可引起大鼠移植肝脏肝内胆管微循环及其内皮细胞功能明显改变,微循环障碍可能在肝内胆管冷保存再灌注损伤中起重要作用。
Objective To discuss the influence of cold preservation on intrahepatic biliary microcirculation Methods Male Sprague Dawley（SD） rats were divided into 3 groups：cold preserve 1 h group（ CP 1 h group） ,cold preserve 24 h group（ CP 24 h group）and sham operation group（ SO group）. Five time points were determined as 0 h,1 h,6 h,24 h and 72 h postoperation. Morphology was observed under light microscope. Dark microspheres were injected into hepatic artery and the number of microspheres in liver portal areas was measured under light microscope. The expressions of eNOS, ET-1 and ICAM-1 in microvascular endothelial cells of hepatic portal area were measured by immunofluorescence double staining technique and in situ hybridization histochemistry. Results The histological changes of intrahepatic bile duct were more severe in CP 24 h group than in CP 1 h group. The number of microspheres in implanted liver portal areas was increased significantly in CP 24 h group than in CP 1 h group at the same time point. Compared with CP 1 h group, the expression of eNOS in CP 24 h group significantly reduced after liver transplantation,while the expressions of ET-1 and ICAM-1 in CP 24 h group were significantly increased after liver transplantatiorL The changes of their mRNA expressions were the approximately same as well as their proteins expressions. Conclusions Cold preservation brings obvious changes of intrahepatic biliary microcirculation and function of vascular endothelial cell after liver transplantation. The obstruction of microcirculation might play an important role in the reperfusion injury after cold preserve of intrahepatic biliary during liver transplantation.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2007年第5期339-343,共5页
Chinese Journal of Surgery
基金
全军医药卫生科研基金科技攻关资助项目(06G071)
关键词
大鼠
肝移植
器官保存
肝内胆管微循环
Rats
Liver transplantation
Organ preservation
Intrahepatic biliary microcirculation
