摘要
以生长在特殊环境中(26℃、12h/12h光/暗周期、光强2500lx)的旱稻297二叶一心期幼苗为材料,构建了干旱胁迫诱导表达差减文库。以干旱为处理,而有水状态为对照。通过筛选差减文库,共获得了141个克隆。分析表明,差减文库建立成功。
Using suppression subtractive hybridization （ SSH）, a forward suppression subtractive cDNA li- brary of drought - regulated gene was constructed in upland rice 297 （ Oryza sativa） seedlings at 2 - leaf stage, by screening the SSH library. The analysis results showed that the subtracted cDNA library had been constructed successfully.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2007年第1期138-141,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家转基因植物研究与产业化专项(J2002-B-004)资助
关键词
早稻
早稻幼苗
干旱
差减文库
upland rice
upland rice seedlings
drought
subtracted library
