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死亡素突变体TH1在裂殖酵母中的分泌表达 被引量：1

Secreting Expression of Thanatin Mutant(TH1) in Schizosaccharomyces Pombe
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摘要 通过PCR方法人工合成带有K28信号肽的死亡素突变体基因,构建了重组表达载体pRHZ-41-k28Th1,转化到裂殖酵母中进行表达,发酵液中检测到与TH1理论分子量一致的多肽分子,微量稀释法检验表达产物具有抑菌活性,并计算了粗产物对4种供试菌的最低抑菌浓度。 To obtain thanatin mutant（TH]） with antibacterial activity for further research, THI cDNA which fused with K28 signal peptide was synthesized by PCR, and ligated to shuttle vector to construct recombinant vector pRHZ-41-KTha 1. This recombinant vector was transformed into yeast cell successfully, and the expressed product was found in the fermental liquid of recombinant yeast ferment liquid by SDS-PAGE electrophoresis. The recombinant product has the antibacterial activity.
作者 文良柱 钱坤 徐寒梅 沈子龙
机构地区 中国药科大学生物技术中心 湖州师范学院生命科学学院
出处 《药物生物技术》 CAS CSCD 2007年第1期18-22,共5页 Pharmaceutical Biotechnology
关键词 死亡素突变体 裂殖酵母 抑菌 Thanatin mutant, Schizosaccharomyces Pornbe, Secreting expression, Antibacteria
分类号 Q786 [生物学—分子生物学]
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参考文献10

  • 1Fehlbaum P,Bulet P,Hoffmann J A.Structure-activity analysis of thanatin,a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides[J].Proc Natl Acad Sci USA,1996,93(3):1221.
  • 2Lee MK,Cha L,Lee SH,Role of amino acid residues within the disulfide loop of thanatin,a potent antibiotic peptide[J].J Biochem Mol Biol,2002,35(3):291.
  • 3Heintel T,Zagorc T,Schmitt MJ,et al.Expression,Processing and high level secretion of a virus toxin in fission yeast[J].Appl Microbiol Biotechnol,2001,56:165.
  • 4Forsburg SL.Comparison of different Schizosaccharomyces pombe expression systems[J].Nucl Acids Res,1993,21:2955.
  • 5Okazaki K,Okazaki N,Kume K,et al.High frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe[J].Nucl Acids Res,1990,18:6485.
  • 6Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophane T4[J].Nature,1970,227:680.
  • 7National Committee for Clinical Laboratory Standards:Performance standards for antimicrobial susceptibility testing approved standard[S].CLSI/NCCLS document M100 -S15,Wayne,1999
  • 8Bonomo R A.Multiple antibiotic resistant bacteria in longterm carefacilities:an emerging problem in the practice of infectious diseases[J].Clin Infect Dis,2000,31:1414.
  • 9钱坤,吕正兵,文良柱,沈子龙.死亡素在裂殖酵母中的表达[J].中国药科大学学报,2005,36(3):276-278. 被引量：4
  • 10Giga -Havia Y,Klumagai H.Foreign Gene Expression in Fission Yeast Schizosaccharomyces pombe[M].Berlin:Springer-Verlag,1997,3.

二级参考文献10

  • 1徐叔云(XUSY) 卞如濂(BianRL) 陈修(ChenX).药理实验方法学[M] 第三版[M].北京:人民卫生出版社,2002.234-235.
  • 2Hancock RE,Lehrer R. Cationic peptides:a new source of antibiotics[J]. Trends Biotedmol, 1998,16:82-88.
  • 3Oren Z, Shai Y. Mode of action of linear amphipathic -helical anfimicrobial DeDfides[J]. Biopolymers. 1998.47: 451-463.
  • 4Fehlbaum P, Bulet P, Chemysh S, et al. Structure-activity analysis of thanatin,a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides[J]. Proc Natl Acad Sci SA, 1996,93(3):1221-1225.
  • 5Mandard N, Sedano P, Labbe H, et al. Solution structure of thanatin, a potent bactericidal and fungicidal insect peptide,determined from proton two-dimensional nuclear magnetic resonance data[J]. Eur J Biochem, 1998,256(2):404-410.
  • 6Forsburg SL. Comparison of different SdUzosacdmromyces pombe expression systems[J]. Nucl Acids Res, 1993,21:2955-2956.
  • 7Joseph Sambrook, David WR, Joe SB. Molecular cloning: A Laboratory Manual[M]. 3 rd Ed, Cold Spring Harbor Lab, 2001.1116-1118.
  • 8Okazaki K, Okazaki N, Kume K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe[J]. Nucl Acids Res, 1990,18:6485-6489.
  • 9Kenneth Robzyk, Yona Kassir, A simple and highly efficient procedure for rescuing autonomous plasmids from yeast [J]. Nucl Acids Res, 1990,20(14):3 790.
  • 10奥斯伯(AoSB) 布伦特(BuRT) 金斯顿(JingSD) 著颜子颖(YanZY) 王海林(WangHL)译.精编分子生物学实验指南[M].北京:科学出版社,1998.771-772.

共引文献3

同被引文献7

  • 1单连慧,时成波,孔双泉,田晓乐,杨亦代,盛军.替换稀有密码子提高HBsAg在CHO细胞中的表达量[J].中国生物制品学杂志,2004,17(4):214-216. 被引量：5
  • 2Fehlbaum P,Bulet P,Chemysh S,et al.Structure activity analysis of thanatin,a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides[J].Proc Natl Acad Sci USA,1996,93(8):1221.
  • 3P.Ramanujam WS,Tan S,Nathan K.Novel peptides that inhibit the propagation of Newcastle disease virus[J].Arch Virol,2002,147(5):981.
  • 4Harada M,Kobayashi K,Matsueda S,et al.Prostate-specific antigen-derived epitopes capable of inducing cellular and humoral responses in HLA-A24 prostate cancer patients[J].Prostate,2003,57 (2):152.
  • 5Wu GQ,Li LX,Ding JX,et al.High-level expression and novel purification strategy of recombinant thanatin analog in Escherichia coli[J].Curr Microbiol,2008,57(2):95.
  • 6谢俊秋,张庆华,张雪燕,姚海燕,韩跃武.抑制新城疫病毒繁殖的九肽及其突变体基因的克隆与原核表达[J].中国生物制品学杂志,2008,21(8):662-664. 被引量：2
  • 7金丰良,张呈文,许小霞,孙强,任顺祥.死亡素与泛素在大肠杆菌中的高效融合表达[J].昆虫学报,2009,52(5):495-501. 被引量：3

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药物生物技术
2007年 第1期

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