摘要
标记基因的产生方便了植物的转化,随着转基因植物的迅速发展及商品化,人类更关注抗性标记基因的安全性。目前解决的有效途径是发展正向选择系统,使用非抗性的生物安全标记基因,主要包括糖类代谢酶基因(pmi和xylA)、干扰氨基酸代谢酶基因(ak和dapA)、绿色荧光蛋白基因(gfp)、β-葡萄糖苷酸酶基因(gus)、核糖醇操纵子(rtl)和叶绿素生物合成基因(hemL)等。
Marker genes have been wildly used for identifying transgenic plants. However, the poor transformation efficiency of most crops necessitates the use of marker genes to identify transgenic plants. Much more concerns are being given to the safety of the resistance marker genes, as the quick development and commercialization of the transgenic plants. Positive selection system has been developed as an efficient alternative by using non-resistance marker genes with biosafety, some of which are sugar metabolic enzyme genes （pmi and xylA ） , disturbing amino acid metabolic enzyme genes （ak and dapA）, green fluorescent protein gene （gfp）,β- glucuronidase gene （gus） , ribitol operon （ rtl）, chlorophyllous biosynthesis enzyme gene （hemL）.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第3期115-119,共5页
China Biotechnology
关键词
转基因植物
抗性标记基因
生物安全
标记基因
正向选择
Transgenic plants Resistance marker genes Biosafety Marker genes Positive selection
