摘要
线粒体是存在于绝大多数真核细胞内的一种基本的、重要的细胞器,是细胞进行氧化磷酸化的场所。不同脊椎动物同源基因序列的比较显示,细胞色素b(Cytb)、细胞色素C氧化酶Ⅰ(COⅠ)、细胞色素C氧化酶Ⅱ(COⅡ)、细胞色素C氧化酶Ⅲ(COⅢ)基因最保守,同源性最高,ATPase6、ATPase8基因、ND基因变异比较大。以家鸭(Anas platyrhynchos)肝脏的线粒体DNA为模板,按照GenBank已经公布的潜鸭族(AF090337)的全序列及其绿头鸭的mtDNA部分序列(L22476、L16770、L22477)设计合成特异引物进行PCR扩增,克隆并测定了线粒体细胞色素C氧化酶Ⅲ亚基(COⅢ)的全序列784bp以及ATPase6基因的3′端和tRNA-Gly基因的5′端序列共934bp。用DNAStar分析软件对家鸭与GenBank中7种禽类的COⅢ序列进行比较分析,显示家鸭与这些动物的COⅢ基因具有较高的同源性,与同科潜鸭属中的Aythya americana的相似性最高为90.6%,与同目不同科的加拿大雁、小天鹅、白额雁的相似性分别为89%、88.6%、88.6%。根据家鸭与其他7种禽类的COⅢ基因序列相似性所建立的进化树,与传统的分类地位基本吻合。
Mitochondrion is the basic and important organelle in most of the eukaryocyte. It is the place of oxidative phosphorylation. The gene sequence of the different vertebrate shows that the gene of cytochrome b （Cytb） ,cytochrome oxidase Ⅰ（COⅠ ） ,cytochrome oxidase Ⅱ （COⅡ ）and cytochrome oxidase Ⅲ（CO Ⅲ ）are the most conservative. The homology is the highest. The genovariation of ATPase6,ATPase8 and ND is much bigger. The mitochondrial cytochrome oxidase Ⅲ（ COⅢ） and its associated tRNA-Gly,ATPase6 genes of domestic duck were amplified by PCR. The open reading frame of COⅢ gene contains 784bp nucleotides, the COⅢ gene of domestic duck shows a high degree of homology with that from the other poultry recorded in the GenBank and it has 90.6% homology with Aythya americana, 89% with Branta canadensis, 88.6% with Anser albifrons and 88. 6% with Cygnus columbianus. The phylogenetic trees show that the relationships based on the homology is consistent with the morphological and taxonomic results.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第3期54-59,共6页
China Biotechnology
关键词
家鸭
细胞色素C氧化酶Ⅲ(COⅢ)
同源性
系统学
Domestic duck
Mitochondrial genome cytochrome oxidaseⅢ（COⅢ ） Homology
molecular evolution
