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SDF-1-pIRES2-EGFP真核表达载体的构建 被引量：4

Construction of eukaryotic expression vector SDF-1-pIRES2-EGFP
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摘要 目的从ATCC提供的、携带有基质细胞衍生因子-1(stromal cell derived factor-1,SDF-1)cDNA的质粒pCMV-SPORT6中获取SDF-1的cDNA全长,并构建SDF-1-pIRES2-EGFP真核表达载体。方法采用双酶切、回收SDF-1cDNA片段,克隆至pIRES2-EGFP真核表达载体,得到SDF-1-pIRES2-EGFP真核表达载体。结果酶切及测序结果表明SDF-1-pIRES2-EGFP真核表达载体构建成功。结论获得SDF-1基因,构建SDF-1-pIRES2-EGFP真核表达载体,为探索SDF-1修饰人骨髓间充质干细胞(Mesenchymal stem cells,MSCs)促进人CD34+细胞归巢及增殖方面的作用奠定基础。 Objective To construct the eukaryotic expression vector of stromal cell derived factor- 1 （ SDF- 1） - pIRES2- EGFP by using full SDF-1cDNA genc obtained from the vector pCMV-SPORT6 which carries the SDF-1cDNA. Methods SDF-lcDNA was acquired from the vector pCMV-SPORT6 through the Xho I / EcoR I endonuclease, and then cloned into eukaryotic expression vector pIRES2-EGFP. The obtained recombinant vector was named SDF-I-pIRES2-EGFP. Results DNA sequencing and restriction endonnclease digestion analysis indicated that the eukaryotic expression vector SDF-I-plRES2-EGFP was constructed successfully. Conclusion The SDF-1cDNA is acquired and the SDF-1-pIRES2-EGFP is constructed successfully, which provide a good experimental basis for further study on the function of SDF-1-modified human bone marrow mesenchymal stem cells to promote the CD34^＋ cells homing and proliferation.
作者 梁雪 粟永萍 孔佩艳 陈幸华 彭贤贵 徐辉 曾东风 艾国平
机构地区 第三军医大学新桥医院血液科 第三军医大学预防医学院全军复合伤研究所
出处 《免疫学杂志》 CAS CSCD 北大核心 2007年第2期152-154,共3页 Immunological Journal
基金 创伤烧伤复合伤国家重点实验室开放基金课题资助(2003)
关键词 基质细胞衍生因子-1 真核表达载体 人骨髓间充质干细胞 Stromal cell derived factor- 1 Eukaryotic expression vector Human bone marrow mesenehymal stem cells
分类号 R346 [医药卫生—基础医学]
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参考文献9

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二级参考文献9

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共引文献9

同被引文献34

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免疫学杂志
2007年 第2期

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