摘要
目的研究肝型脂肪酸结合蛋白(L-FABP)与血管内皮生长因子(VEGF)在原发性肝癌中的表达变化,探讨二者在原发性肝癌发生、发展中的作用及相互关系。方法采用RT-PCR及免疫组织化学技术,检测61例手术切除的病人肝癌组织及癌旁组织中L-FABP、VEGF的mRNA及蛋白质的表达水平,并做统计学处理。结果RT-PCR结果显示,肝癌L-FABP以及VEGF在mRNA表达水平明显高于癌旁组织(L-FABP:0.97±0.12,0.83±0.14,t=5.21,P<0.05;VEGF:0.92±0.11,0.59±0.15,t=11.79,P<0.05)。免疫组化染色发现,L-FABP阳性反应物主要存在于细胞浆,肝癌组阳性反应物灰度值明显高于癌旁对照组(肝癌组:92.73±7.67,癌旁对照组:82.83±6.90,t=7.44,P<0.05),阳性细胞计数肝癌组高于癌旁对照组(肝癌组:92.18±4.44,癌旁对照组:84.52±6.43,t=5.94,P<0.05);肝癌细胞胞浆有VEGF阳性反应物,在癌旁组织细胞则偶见VEGF阳性反应物,差异有显著性(肝癌组:88.69±5.56,癌旁对照组:77.61±5.93,t=8.72,P<0.05)。相关性分析表明L-FABP以及VEGF在肝癌组织与癌旁组织中在mRNA和蛋白质水平上均有正相关性(P<0.05)。结论L-FABP、VEGF基因以及蛋白质在肝癌组织中表达均显著上调,并且二者具有正相关性,我们推测L-FABP在获取更多的脂肪酸的同时可以促进血管的增生,进而促进了肝癌的发展。
Objective To detect the expression of liver-type fatty acid-binding protein （L-FABP） and vascular endothelial growth factor （VEGF） in human hepatocelhilar carcinoma （HCC） and its adjacent liver tissues, and investigate the correlation between the expressions of L-FABP and VEGF and their role in the occurrence and progression of lice. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemical （IHC） staining were employed to examine the expression of L-FABP and VEGF in HCC and its adjacent liver tissues obtained from the surgical specimens of 61 HCC patients who underwent liver resections in West China hospital. Results The results of RT-PCR showed that the expression level of L-FABP and VEGF in HCC was significantly higher than that in its adjacent liver tissues （L-FABP： 0.97±0.12, 0.83± 0.14, t=5.21, P〈0.05; VEGF： 0.92±0.11, 0.59±0.15, t=11.79, P〈0.05）. L-FABP tended to co-express with VEGF （P〈0.05）. IHC staining revealed that the expression of L-FABP and VEGF was mainly located in the cytoplasm, and the gray scale of L-FABP expression was significantly higher than that in the adjacent liver tissues （92.73±7.67, 82.83±6.90, t=7.44, P〈0.05）. The number of L-FABP- and VEGF-positive cells in HCC was significantly lower than that in the adjacent liver tissues （L-FABP： 92.18±4.44, 84.52±6.43, t=5.94, P〈0.05; VEGF： 88.69±5.56, 77.64±5.93, t=8.72, P〈0.05）. Co-expression of L-FABP and VEGF observed in RT-PCR and also in IHC （P〈0.05）. Conclusion Both L-FABP and VEGF expressions are up-regulated in HCC. L-FABP gene may be involved in the carcinogenesis of human HCC. Expression of L-FABP is associated with VEGF expression, suggesting that L-FABP promotes the growth of blood vessels by taking up the fatty acids from the bloodstream, and both of them produce a marked effect on energy metabolism in HCC.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第3期318-321,共4页
Journal of Southern Medical University
基金
四川大学青年科学基金(060594)~~
关键词
脂肪酸结合蛋白
血管内皮生长因子
原发性肝癌
RT-PCR
免疫组化
fatty acid-binding protein
vascular endothelial growth factor
primary hepatocellular carcinoma
reversetranscriptase-polymerase chain reaction
immunohistochemistry
