摘要
目的研究骨髓间充质干细胞与肝癌细胞药敏与肝癌细胞成瘤能力的相关性。方法30只雌性清洁级SD大鼠应用二乙基亚硝胺饲喂法建立大鼠原发性肝癌模型,选取诱癌成功大鼠10只分别原代培养肿瘤细胞与间充质干细胞,MTT法用于不同作用机制抗肿瘤药物的药物敏感实验,药物作用后接种至裸鼠,观察其成瘤实验的差异,对模型大鼠间充质干细胞、肿瘤细胞抑制率与化疗药物作用后肿瘤细胞移植裸鼠后的成瘤质量分别进行相关性分析。结果5种药物药敏结果显示肝癌癌细胞与间充质干细胞药物敏感无明显相关性(P<0.05)。间充质细胞药敏实验与成瘤实验正相关。结论间充质干细胞耐药机制与肿瘤干细胞相似,有希望代替普通肝癌肿瘤细胞进行药物敏感实验。
Objective To study the association of drug sensitivity of rot hepatocarcinoma cells and bone marrow mesenchymal stem cells （MSCs） to 5 antitumor drug with different antitumor mechanisms with tumorigenicity of the hepatocarcinoma cells in nude mice. Methods Primary liver carcinoma was induced with diethylnitrosamine in rats, and the tumor cells and MSCs were obtained fi＇om 10 of the rats. The inhibition ratio of the hepatocarcinoma cells and MSCs following treatment with the 5 drugs were measured by MTT assay. The weight of the tumor in nude mice resulting fi＇om the injection of the isolated tumor cells treated with the 5 anticancer drugs was measured 6 weeks after implantation. For each anticancer drug, the difference in the inhibition ratio of the anticancer drugs against the hepatocarcinoma cells and MSCs, and he correlation of the inhibition ratio of the anticancer drugs with implanted tumor weight were analyzed. Results No correlation was found between the inhibition ratio of the 5 anticancer drugs against the hepatocarcinoma cells and the tumor weight of nude mice, but a significant negative correlation was identified between the inhibition ratio of the MSCs and the tumor weight. Conclusion MSCs have similar drug resistance mechanism to the tumor stem cells. The inhibition ratio of the anticancer drugs against the MSCs can help evaluate the invasion potential of hepatocarcinoma cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第3期290-292,共3页
Journal of Southern Medical University
基金
国家自然科学基金(30572152)~~
关键词
肝肿瘤
肿瘤干细胞
间充质干细胞
抗肿瘤药物
药物敏感实验
live, neoplasms
tumor stem cells
mesenchymal stem cells
anfitumor drugs
drug sensitivity assay
