摘要
目的建立离子色谱法测尿碘,以便能应用于流行病学调查研究。方法选用ICS-2500型离子色谱仪、IonPacAS7(4mm×250mm)分析柱和银电极,在直流安培模式下测尿碘。应用人群选择:有自理能力的广州尊老康乐协会50岁以上会员569名,测尿碘、甲状腺刺激素和游离甲状腺素,并调查甲状腺病病史。结果(1)离子色谱峰面积与尿碘浓度呈线性相关,相关系数为0.9999,最低检出限约2.2μg/L,变异系数3.16%～3.45%,平均回收率95.0%;(2)在467名合格的入选对象中,尿碘浓度呈正偏态分布,中位数为224.7μg/L。结论采用的离子色谱法测尿碘选择性好、灵敏、快速、可信、准确、稳定,应用于人群效果满意。
Objective To establish a method of ion chromatography for urine iodide determination and evaluate its application in cpidemiological studies. Methods Urinary iodine was determined using ICS-2500 ion chromatography coupled with IonPac AS7 analytical column （4 min×250 mm）, silver electrode and direct current amperometry for 467 elderly subjects （above 50 years） with their urinary Tsh and FT4 also determined. The history of thyroid diseases was examined among these subjects. Results The peak area of the ion chromatography was linearly correlated with urinary iodine concenlration, with the correlation coefficient of 0.9999. The detection limit of iodide was around 3.5 μg/L （S/N=3）, with coefficients of variation ranging from 3.16% to 3.45% and mean recovery rote of 95%. The urinary iodine level in the 467 elderly subjects showed a positively skewed distribution with the median level of 224.7 μg/L. Conclusion Ion chromatography for urinary iodine determination has excellent selectivity, sensitivity, reliability, accuracy and stability, and may ensure satisfactory effect for application in cpidemiological studies.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第3期286-289,共4页
Journal of Southern Medical University
基金
广州市科技局项目(2002Z-E025)
广州市卫生局项目
香港大学教育与科学基金会
英国伯明翰大学资助项目~~
