摘要
目的了解糖尿病大鼠白膜中弹性纤维改变对勃起功能的影响。方法利用链脲佐菌素(STZ)制作糖尿病及糖尿病性勃起功能障碍模型,40只SPF级SD雄性大鼠,先按时间随机分为两组(每组20只),分别是注射STZ后7周组和4周组,再按处理因素即给予大鼠腹腔注射STZ(60mg/kg)是否成模,分为对照组(未注射STZ,5只)、糖尿病性勃起功能障碍模型组、糖尿病性非勃起功能障碍组、未成模组。利用维多利亚蓝-丽春红染色法对不同处理组的标本进行弹性纤维染色,利用彩色图文系统进行分析,以累积光密度(IOD)作为测量指标。结果各处理组间IOD存在显著性差异(F=10.433,P<0.001),糖尿病性勃起功能障碍组IOD均小于对照组、糖尿病非勃起功能障碍组及未成模组(P<0.05),糖尿病非勃起功能障碍组的IOD小于对照组(P<0.05),不同时间点间的IOD不存在显著性差异(F=0.685,P=0.415),不存在交互效应(F=0.905,P=0.452)。结论糖尿病可以导致白膜弹性纤维减少;白膜中的弹性纤维在勃起过程中起重要作用;白膜中弹性纤维的减少将导致勃起功能障碍。
Objective To investigate the effect of elastic fiber alterations in the tunica albuginea of the penis on erectile function of diabetic rats. Methods Streptozotocin （STZ） injection was adopted to produce rat models of diabetes mellitus and erectile dysfunction. Forty rats were randomized equally into two groups according to the time after streptozotocin （STZ） injection, namely 4 week group and 7 week group. Each group was further divided into 4 subgroups, including a control group （n=5, without STZ injection）, diabetic with erectile dysfunction group （DM＆ED group）, diabetic without erectile dysfunction group （DM group） and group with neither diabetes mellitus or erectile dysfunction after STZ injection （None group）. Victoria blue/Ponceau red staining and color image analysis were used to observe the content of the elastic fibers in the tunica albuginea, which was quantified by means of integrated optical density （IOD） readings. Result Significant difference in the IOD was observed between different groups （F=10.433, P〈0.001）. The content of elastic fibers in the tunica albuginea was the lowest in DM＆ED group among the 4 groups （P〈0.05）, and there was no significant difference between 7-week and 4 week groups （F=0.685, P=0.415）, nor was any interaction observed （F=0.905, P=0.452）. Conclusions Decreased elastic fibers in the tunica albuginea can result from diabetes mellitus. Elastic fibers in the tunica albuginea play an important role in the course of erection, and erectile dysfunction may result from decreased elastic fiber content.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第3期276-278,共3页
Journal of Southern Medical University
基金
全军十五科技计划基金(03M105)
广东省自然科学基金(5B1040144)~~
关键词
糖尿病
勃起功能障碍
阴茎
白膜
弹性纤维
模型
动物
diabetes mellitus
erectile dysfunction
penis
tunica albuginea
elastic fiber
models, animal
