摘要
The aim of this study was to associate the generation of reactive oxygen species （ROS） with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-（N）, phosphorus-（P）, or potassium-（K） deftcient mulberry （Morus alba L. var. Kanva-2） plants. The indicators of oxidative stress and cellular redox environment and antioxldant defense-related parameters were analyzed. Oeficlency of N, P or K suppressed growth, accelerated senescence, and decreased concentrations of chloroplastic pigments and glutathione. Lipid peroxidation and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase were also increased in these N, P, or K deprived plants. Concentration of hydrogen peroxide Increased in plants deficient in N or P. Oeficlency of N or P particularly altered the cellular redox environment as indicated by changes in the redox couples, namely ascorbic acid/total ascorbate decreased in P-, glutathione sulfydryl/total glutathione decreased in N-, and Increased in P-deficient plants. Activity staining of native gels for superoxide dismutase revealed Increased activity as Indicated by Increased intensity of bands, and induction of few new isoforms in P- and K-deficient plants. Oifferences in the patterns of superoxide dismutase isoforms and redox status （ascorbic acid/total ascorbate and glutathlone sulfydryl/total glutathione） Indicate that N-, P-, or K-deficiency altered antioxidant responses to varying extents in mulberry plants.
The aim of this study was to associate the generation of reactive oxygen species （ROS） with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-（N）, phosphorus-（P）, or potassium-（K） deftcient mulberry （Morus alba L. var. Kanva-2） plants. The indicators of oxidative stress and cellular redox environment and antioxldant defense-related parameters were analyzed. Oeficlency of N, P or K suppressed growth, accelerated senescence, and decreased concentrations of chloroplastic pigments and glutathione. Lipid peroxidation and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase were also increased in these N, P, or K deprived plants. Concentration of hydrogen peroxide Increased in plants deficient in N or P. Oeficlency of N or P particularly altered the cellular redox environment as indicated by changes in the redox couples, namely ascorbic acid/total ascorbate decreased in P-, glutathione sulfydryl/total glutathione decreased in N-, and Increased in P-deficient plants. Activity staining of native gels for superoxide dismutase revealed Increased activity as Indicated by Increased intensity of bands, and induction of few new isoforms in P- and K-deficient plants. Oifferences in the patterns of superoxide dismutase isoforms and redox status （ascorbic acid/total ascorbate and glutathlone sulfydryl/total glutathione） Indicate that N-, P-, or K-deficiency altered antioxidant responses to varying extents in mulberry plants.
基金
Supported by the Council of Science and Technology, U.P., Lucknow and the Council of Scientific and Industrial Research （CSIR）, New Delhi, India. Publication of this paper is supported by the National Natural Science Foundation of China （30624808） and Science Publication Foundation of the Chinese Academy of Sciences.Acknowledgements We thank Dr P. K. Singh for his assistance and for providing AAS facilities.
