摘要
目的研究短期血浆游离脂肪酸（FFA）升高对大鼠肝脏和外周胰岛素作用的影响，比较不同饱和程度脂肪酸对大鼠肝脏和外周胰岛素抵抗的作用。方法分别给大鼠静脉输注脂肪乳（多不饱和脂肪酸）＋肝素，油酸（OLE，单不饱和脂肪酸），猪油（LO，饱和脂肪酸）＋肝素（LOH组）和生理盐水7h，并在输注的最后2h，行清醒状态高胰岛素．正血糖钳夹试验，测定血浆葡萄糖、FFA、胰岛素和c肽水平，观察不同脂肪酸对胰岛素介导的葡萄糖利用（GU）和肝葡萄糖生成（HGP）能力的影响。结果与生理盐水组比较，脂肪乳、OLE和LOH组血浆FFA水平升高（均P〈0．01）。在钳夹状态下，所有脂肪组的HGP高于生理盐水组（均P〈0．01），而OLE组和LOH组的HGP明显高于脂肪乳组（均P〈0．01），所有脂肪组GU下降（均P〈0．01），但是不同脂肪组间GU差异无统计学意义。结论血浆脂肪酸短期升高能引起大鼠肝脏和外周胰岛素抵抗，多不饱和脂肪酸引起的肝胰岛素抵抗小于单不饱和脂肪酸或饱和脂肪酸，不同饱和程度脂肪酸诱导的周围胰岛素抵抗程度相似。
Objective To investigate the effect of short-term elevation of plasma free fatty acids （FFA） on hepatic and peripheral insulin action and to determine whether FFA with different degrees of unsaturation have different effects on hepatic and peripheral insulin resistance. Methods Intralipid ＋ Heparin （ IH, polyunsaturated fatty acids）, Oleate （OLE, monounsaturated fatty acids）, Lard Oil ＋ Heparin （LOH, saturated fatty acids）, and saline （SAL） were intravenously infused for 7 h in rats. During the last 2 h of the infusion, a hyperinsulinemiceuglycemic clamp was performed to measure plasma glucose, FFA, insulin and C-peptide, and to examine the ability of insulin to stimulate glucose utilization （ GU ） and suppress hepatic glucose production （HGP）. Results Compared with SAL, there were elevated plasma FFA levels in rats treated with IH, OLE and LOH infusions （all P 〈0.01）. All three fat infusion groups had remarkablely higher HGP than SAL group （all P 〈0. 01 ）. OLE and LOH infusions induced much higher HGP than IH （ both P 〈 0.01 ）. GU was decreased in all three fat infusion groups compared with SAL group （ all P 〈0.01 ）, but there were no significant difference among different types of fat infusions. Conclusion Short-term elevation of plasma FFA may induce hepatic and peripheral insulin resistance in rats. Polyunsaturated fatty acids induced less hepatic insulin resistance than monounzaturated or saturated fatty acids. However, IH, OLE and LOH infusions induced similar peripheral insulin resistance.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2007年第1期16-19,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
脂肪酸类
胰岛素抵抗
肝葡萄糖生成
葡萄糖利用
Free fatty acids
Insulin resistance
Hepatic glucose production
Glucose utilization
