摘要
目的观察人参多糖和猪苓多糖对大鼠肠道黏膜淋巴细胞功能的影响。方法分离SD大鼠外周血单个核细胞(PBMC)、派伊尔结淋巴细胞(PPL)、肠道上皮内淋巴细胞(IEL)和黏膜固有层淋巴细胞(LPL),分别与不同质量浓度的人参多糖和猪苓多糖共同培养,检测上清液中肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)水平变化。结果人参多糖和猪苓多糖均可以使PBMC和PPL培养上清液中TNF-α和IFN-γ水平升高;2mg/mL猪苓多糖可以使IEL培养上清液中TNF-α和IFN-γ水平升高;人参多糖和猪苓多糖可以使LPL培养上清液中TNF-α和IFN-γ水平降低。结论人参多糖和猪苓多糖对大鼠肠道黏膜淋巴细胞功能具有调节作用。
Objective To explore the effect of ginseng polysaccharide and Polyporus umbeUatus polysaccharide on function of T-lymphocytes in enteric mucosa of rats. Methods The peripheral blood mononuclear cells （PBMC）, Peyer＇s Patche lymphocyte （PPL）, intraepithelial lymphocyte （IEL）, and lamina propria lymphocyte （LPL） of SD rats were isolated. These lymphocytes were co-cultured with ginseng polysaccharide and P. umbellatus polysaccharide of different concentrations. The changes of TNF-α and IFN-γ level in supernatants were observed with ELISA. Results Ginseng polysaccharide and P. umbellatus polysaccharide increased the level of TNF-α and IFN-γ in the supernatants of PBMC and PPL; P. umbellatus polysaccharide in the dosage of 2 mg/mL increased the level of TNF-α and IFN-γ in supernatant of IEL; ginseng polysaccharide and P. umbellatus polysaccharide decreased the level of TNF-α and IFN-γ in supernatant of LPL. Conclusion Ginseng polysaccharide and P. umbeUatus polysaccharide can regulate the function of lymphocytes in enteric mucosal immune system.
出处
《中草药》
CAS
CSCD
北大核心
2007年第2期221-224,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30171133)
关键词
人参多糖
猪苓多糖
肠道黏膜免疫
淋巴细胞
ginseng polysaccharide
Polyporus umbellatus polysaccharide
intestinal mucosal immunity
lymphocyte
