摘要
目的利用微生物转化法对人参果总皂苷(SFPG)进行生物转化,制备人参皂苷化合物K(C-K)。方法从种植人参的土壤中筛选、分离4种微生物m14、m3、m8、m9对SFPG进行转化,筛选最佳活性菌株;以C-K的量为指标,采用TLC和HPLC法检测微生物的转化结果。结果真菌m14为转化C-K的最佳活性菌株,最佳转化条件为转化时间6d,转化温度30℃,摇床转数160r/min,转化初始pH值为自然pH5.5,转化底物质量浓度为120mg/mL。转化后的C-K的量是转化前的41.65倍。结论真菌m14转化SFPG中C-K的专属性强、转化效率高,为C-K的工业化生产提供了一条新的途径。
Objective To apply the microbial transformation to transforming the total saponins in the fruit of Panax ginseng （SFPG） and preparing ginsenoside compound K （C-K）. Methods The four microbial strains m14, m3, m8, and m9 were screened and isolated from the soil in the botanic garden planted for P. ginseng and they were used for the microbial transformation of SFPG to optimize the strains. Taking C-K contents as index the microbial transformation was detected and analyzed by TLC and HPLC. Results The strain m14 was found to transform the SFPG efficiently to C-K at first. The optimal culturing and transformation conditions of m14 were obtained ： time, 6 d ; temperature, 30 ℃ ; revolution of cradle, 160 r/min; initial pH value, 5. 5; substratum concentration, 120 mg/mL. Under the optimal condition, the content of C-K was 41.65 times as much as before transformation by m14. Conclusion The m14 is the most effective strain among the four fungal strains. It is the new way available for the C-K industrialized production.
出处
《中草药》
CAS
CSCD
北大核心
2007年第2期189-193,共5页
Chinese Traditional and Herbal Drugs
基金
辽宁省自然科学基金资助项目(20062031
20062069)
关键词
人参果总皂苷(SFPG)
人参皂苷化合物K(C-K)
菌株m14
微生物转化
the total saponins in the fruit of Panax ginseng C. A. Meyer （SFPG）
ginsenoside compound K （C-K）
strain m14
microbial transformation
